Proteome reference map of the skin mucus of Atlantic cod (Gadus morhua) revealing immune competent molecules

B. Rajan, J.M.O. Fernandes, C.M.A. Caipang, V. Kiron, J.H.W.M. Rombout, M. Brinchmann

Research output: Contribution to journalArticleAcademicpeer-review

98 Citations (Scopus)

Abstract

The skin mucosal proteome of Atlantic cod (Gadus morhua) was mapped using a 2D PAGE, LC–MS/MS coupled approach. Mucosal proteins from naive fish were identified primarily by similarity searches across various cod EST databases. The identified proteins were clustered into 8 groups based on gene ontology classification for biological process. Most of the proteins identified from the gel are hitherto unreported for cod. Galectin-1, mannan binding lectin (MBL), serpins, cystatin B, cyclophilin A, FK-506 binding protein, proteasome subunits (alpha-3 and -7), ubiquitin, and g-type lysozyme are considered immune competent molecules. Five of the aforementioned proteins were cloned and their tissue distribution was analysed by RT-PCR
Original languageEnglish
Pages (from-to)224-231
JournalFish and Shellfish Immunology
Volume31
Issue number2
DOIs
Publication statusPublished - 2011

Keywords

  • channel catfish
  • epidermal mucus
  • lepeophtheirus-salmonis
  • ictalurus-punctatus
  • neutrophil elastase
  • intestinal mucus
  • conger-myriaster
  • cyprinus-carpio
  • binding-lectin
  • cyclophilin-a

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