Proteome reference map of the skin mucus of Atlantic cod (Gadus morhua) revealing immune competent molecules

B. Rajan; J.M.O. Fernandes; C.M.A. Caipang; V. Kiron; J.H.W.M. Rombout; M. Brinchmann

doi:10.1016/j.fsi.2011.05.006

Proteome reference map of the skin mucus of Atlantic cod (Gadus morhua) revealing immune competent molecules

B. Rajan, J.M.O. Fernandes, C.M.A. Caipang, V. Kiron, J.H.W.M. Rombout, M. Brinchmann

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The skin mucosal proteome of Atlantic cod (Gadus morhua) was mapped using a 2D PAGE, LC–MS/MS coupled approach. Mucosal proteins from naive fish were identified primarily by similarity searches across various cod EST databases. The identified proteins were clustered into 8 groups based on gene ontology classification for biological process. Most of the proteins identified from the gel are hitherto unreported for cod. Galectin-1, mannan binding lectin (MBL), serpins, cystatin B, cyclophilin A, FK-506 binding protein, proteasome subunits (alpha-3 and -7), ubiquitin, and g-type lysozyme are considered immune competent molecules. Five of the aforementioned proteins were cloned and their tissue distribution was analysed by RT-PCR

Original language	English
Pages (from-to)	224-231
Journal	Fish and Shellfish Immunology
Volume	31
Issue number	2
DOIs	https://doi.org/10.1016/j.fsi.2011.05.006
Publication status	Published - 2011

Keywords

channel catfish
epidermal mucus
lepeophtheirus-salmonis
ictalurus-punctatus
neutrophil elastase
intestinal mucus
conger-myriaster
cyprinus-carpio
binding-lectin
cyclophilin-a

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10.1016/j.fsi.2011.05.006

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title = "Proteome reference map of the skin mucus of Atlantic cod (Gadus morhua) revealing immune competent molecules",

abstract = "The skin mucosal proteome of Atlantic cod (Gadus morhua) was mapped using a 2D PAGE, LC–MS/MS coupled approach. Mucosal proteins from naive fish were identified primarily by similarity searches across various cod EST databases. The identified proteins were clustered into 8 groups based on gene ontology classification for biological process. Most of the proteins identified from the gel are hitherto unreported for cod. Galectin-1, mannan binding lectin (MBL), serpins, cystatin B, cyclophilin A, FK-506 binding protein, proteasome subunits (alpha-3 and -7), ubiquitin, and g-type lysozyme are considered immune competent molecules. Five of the aforementioned proteins were cloned and their tissue distribution was analysed by RT-PCR",

keywords = "channel catfish, epidermal mucus, lepeophtheirus-salmonis, ictalurus-punctatus, neutrophil elastase, intestinal mucus, conger-myriaster, cyprinus-carpio, binding-lectin, cyclophilin-a",

author = "B. Rajan and J.M.O. Fernandes and C.M.A. Caipang and V. Kiron and J.H.W.M. Rombout and M. Brinchmann",

year = "2011",

doi = "10.1016/j.fsi.2011.05.006",

language = "English",

volume = "31",

pages = "224--231",

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T1 - Proteome reference map of the skin mucus of Atlantic cod (Gadus morhua) revealing immune competent molecules

AU - Rajan, B.

AU - Fernandes, J.M.O.

AU - Caipang, C.M.A.

AU - Kiron, V.

AU - Rombout, J.H.W.M.

AU - Brinchmann, M.

PY - 2011

Y1 - 2011

N2 - The skin mucosal proteome of Atlantic cod (Gadus morhua) was mapped using a 2D PAGE, LC–MS/MS coupled approach. Mucosal proteins from naive fish were identified primarily by similarity searches across various cod EST databases. The identified proteins were clustered into 8 groups based on gene ontology classification for biological process. Most of the proteins identified from the gel are hitherto unreported for cod. Galectin-1, mannan binding lectin (MBL), serpins, cystatin B, cyclophilin A, FK-506 binding protein, proteasome subunits (alpha-3 and -7), ubiquitin, and g-type lysozyme are considered immune competent molecules. Five of the aforementioned proteins were cloned and their tissue distribution was analysed by RT-PCR

AB - The skin mucosal proteome of Atlantic cod (Gadus morhua) was mapped using a 2D PAGE, LC–MS/MS coupled approach. Mucosal proteins from naive fish were identified primarily by similarity searches across various cod EST databases. The identified proteins were clustered into 8 groups based on gene ontology classification for biological process. Most of the proteins identified from the gel are hitherto unreported for cod. Galectin-1, mannan binding lectin (MBL), serpins, cystatin B, cyclophilin A, FK-506 binding protein, proteasome subunits (alpha-3 and -7), ubiquitin, and g-type lysozyme are considered immune competent molecules. Five of the aforementioned proteins were cloned and their tissue distribution was analysed by RT-PCR

KW - channel catfish

KW - epidermal mucus

KW - lepeophtheirus-salmonis

KW - ictalurus-punctatus

KW - neutrophil elastase

KW - intestinal mucus

KW - conger-myriaster

KW - cyprinus-carpio

KW - binding-lectin

KW - cyclophilin-a

U2 - 10.1016/j.fsi.2011.05.006

DO - 10.1016/j.fsi.2011.05.006

M3 - Article

SN - 1050-4648

VL - 31

SP - 224

EP - 231

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

IS - 2

ER -

Proteome reference map of the skin mucus of Atlantic cod (Gadus morhua) revealing immune competent molecules

Abstract

Keywords

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