Protein interactions of MADS box transcription factors involved in flowering in Lolium perenne

S. Ciannamea, K. Kaufmann, M. Frau, I.A. Nougalli Tonaco, K. Petersen, K.K. Nielsen, G.C. Angenent, G.H. Immink

Research output: Contribution to journalArticleAcademicpeer-review

41 Citations (Scopus)

Abstract

Regulation of flowering time is best understood in the dicot model species Arabidopsis thaliana. Molecular analyses revealed that genes belonging to the MADS box transcription factor family play pivotal regulatory roles in both the vernalization- and photoperiod-regulated flowering pathways. Here the analysis of three APETALA1 (AP1)-like MADS box proteins (LpMADS1¿3) and a SHORT VEGETATIVE PHASE (SVP)-like MADS box protein (LpMADS10) from the monocot perennial grass species Lolium perenne is reported. Features of these MADS box proteins were studied by yeast two-hybrid assays. Protein¿protein interactions among the Lolium proteins and with members of the Arabidopsis MADS box family have been studied. The expression pattern for LpMADS1 and the protein properties suggest that not the Arabidopsis AP1 gene, but the SUPPRESSOR OF CONSTANS1 (SOC1) gene, is the functional equivalent of LpMADS1. To obtain insight into the molecular mechanism underlying the regulation of LpMADS1 gene expression in vernalization-sensitive and -insensitive Lolium accessions, the upstream sequences of this gene from a winter and spring growth habit variety were compared with respect to MADS box protein binding. In both promoter elements, a putative MADS box transcription factor-binding site (CArG-box) is present; however, the putative spring promoter has a short deletion adjacent to this DNA motif. Experiments using yeast one-hybrid and gel retardation assays demonstrated that the promoter element is bound by an LpMADS1¿LpMADS10 higher order protein complex and, furthermore, that this complex binds efficiently to the promoter element from the winter variety only. This strongly supports the model that LpMADS1 together with LpMADS10 controls the vernalization-dependent regulation of the LpMADS1 gene, which is part of the vernalization-induced flowering process in Lolium
Original languageEnglish
Pages (from-to)3419-3431
JournalJournal of Experimental Botany
Volume57
Issue number13
DOIs
Publication statusPublished - 2006

Keywords

  • arabidopsis fruit-development
  • floral inductive pathways
  • homeotic gene
  • dna-binding
  • vernalization response
  • reproductive growth
  • transition
  • wheat
  • expression
  • apetala1

Fingerprint

Dive into the research topics of 'Protein interactions of MADS box transcription factors involved in flowering in Lolium perenne'. Together they form a unique fingerprint.

Cite this