TY - JOUR
T1 - Protective effects of green tea polyphenols against benzo[a]pyrene-induced reproductive and trans-generational toxic effects in Japanese Medaka (Oryzias latipes)
AU - Song, Chuankui
AU - Wang, Yanli
AU - Xiao, Zhengcao
AU - Xiao, Bin
PY - 2015
Y1 - 2015
N2 - In order to investigate the protective effect of green tea polyphenols (GTP) on benzo[a]pyrene (BaP)-induced reproductive and trans-generational toxicity, Japanese Medaka was injected intraperitoneally with BaP alone and co-injected with both BaP and GTP of different concentrations, respectively. Exposure to BaP alone significantly suppressed fecundity, fertilization success, and egg diameter/protein content and markedly induced deformation ratio in F1 generation. However, GTP could help recover reproductive functions, egg quality suppressed by BaP and reduced deformation ratio. VTG-1, a molecular marker of reproductive toxicity, was significantly down-regulated in females exposed to BaP, but a dose-dependent increase was observed in co-exposed groups. Up-regulation of GSH-Px, dose-dependent decrease of CYP1A1, and the GST expression inhibited by BaP was recovered and even markedly up-regulated in the co-exposure groups. These findings show promising protective effects of GTP against BaP-induced reproductive and trans-generational toxicity.
AB - In order to investigate the protective effect of green tea polyphenols (GTP) on benzo[a]pyrene (BaP)-induced reproductive and trans-generational toxicity, Japanese Medaka was injected intraperitoneally with BaP alone and co-injected with both BaP and GTP of different concentrations, respectively. Exposure to BaP alone significantly suppressed fecundity, fertilization success, and egg diameter/protein content and markedly induced deformation ratio in F1 generation. However, GTP could help recover reproductive functions, egg quality suppressed by BaP and reduced deformation ratio. VTG-1, a molecular marker of reproductive toxicity, was significantly down-regulated in females exposed to BaP, but a dose-dependent increase was observed in co-exposed groups. Up-regulation of GSH-Px, dose-dependent decrease of CYP1A1, and the GST expression inhibited by BaP was recovered and even markedly up-regulated in the co-exposure groups. These findings show promising protective effects of GTP against BaP-induced reproductive and trans-generational toxicity.
KW - Benz[a]pyrene
KW - Deformation
KW - Green tea polyphenols
KW - Japanese Medaka
KW - Reproduction toxicity
U2 - 10.1016/j.jff.2015.01.028
DO - 10.1016/j.jff.2015.01.028
M3 - Article
AN - SCOPUS:84926294118
SN - 1756-4646
VL - 14
SP - 354
EP - 362
JO - Journal of Functional Foods
JF - Journal of Functional Foods
ER -