Protective antiviral immune responses to pseudorabies virus induced by DNA vaccination using dimethyldioctadecylammonium bromide as an adjuvant

E.M.A. van Rooij, H.L. Glansbeek, L.A.T. Hilgers, E.G. te Lintelo, Y.E. de Visser, W.J.A. Boersma, B.L. Haagmans, A.T.J. Bianchi

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    Abstract

    To enhance the efficacy of a DNA vaccine against pseudorabies virus (PRV), we evaluated the adjuvant properties of plasmids coding for gamma interferon or interleukin-12, of CpG immunostimulatory motifs, and of the conventional adjuvants dimethyldioctadecylammonium bromide in water (DDA) and sulfolipo-cyclodextrin in squalene in water. We demonstrate that a DNA vaccine combined with DDA, but not with the other adjuvants, induced significantly stronger immune responses than plasmid vaccination alone. Moreover, pigs vaccinated in the presence of DDA were protected against clinical disease and shed significantly less PRV after challenge infection. This is the first study to demonstrate that DDA, a conventional adjuvant, enhances DNA vaccine-induced antiviral immunity.
    Original languageEnglish
    Pages (from-to)10540-10545
    JournalJournal of Virology
    Volume76
    DOIs
    Publication statusPublished - 2002

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  • Cite this

    van Rooij, E. M. A., Glansbeek, H. L., Hilgers, L. A. T., te Lintelo, E. G., de Visser, Y. E., Boersma, W. J. A., Haagmans, B. L., & Bianchi, A. T. J. (2002). Protective antiviral immune responses to pseudorabies virus induced by DNA vaccination using dimethyldioctadecylammonium bromide as an adjuvant. Journal of Virology, 76, 10540-10545. https://doi.org/10.1128/JVI.76.20.10540-10545.2002