Protection of in-vitro grown Arabidopsis seedlings against abiotic stresses

G.J.M. de Klerk, P. Pumisutapon

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)


Severe abiotic stresses may cause permanent damage leading to death. In Arabidopsis seedlings germinating in vitro, we examined whether stress-related damage could be reduced by addition of protective low-molecular-weight compounds (trehalose and putrescine), addition of a specific signal molecule (acetylsalicylic acid), culture in the dark before and/or after the stress, and hardening mild-stress pretreatments. All four tested protective procedures increased survival after exposure to drought, warm air, warm water or salinity, often from ca. 15% in the control to ca. 85% in the treated seedlings. These protective measures may be usable to reduce the negative effects of abiotic stresses related to tissue culture.
Original languageEnglish
Pages (from-to)149-154
JournalPlant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants
Issue number2
Publication statusPublished - 2008


  • plants
  • tolerance
  • thermotolerance
  • trehalose
  • proteins
  • drought
  • biosynthesis
  • accumulation
  • performance
  • expression


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