Hydrogen can be produced through dark anaerobic fermentation using carbohydrate-rich biomass, and through photofermentation using the organic acids produced from dark fermentation. Sugar beet is an ideal energy crop for fermentative production of hydrogen in the EU due to its environmental profile and its potential availability in the area. In this work, various aspects of cultivating sugar beet in the EU for biohydrogen were highlighted, with special focus on The Netherlands and Greece. Moreover, fermentation of sugar beet juice with Caldicellulosiruptor saccharolyticus at sucrose concentration 10 g/l was performed, and was found comparable to the fermentation on pure sucrose except that the hydrogen production was 10% higher on sugar beet juice. A conservative estimate of the annual hydrogen potential in the EU was made (300 × 106 kg hydrogen), considering the utilization of sugar beet pulp in hydrogen production.
Panagiotopoulos, I. A., Bakker, R. R., de Vrije, G. J., Urbaniec, K., Koukios, E. G., & Claassen, P. A. M. (2010). Prospects of utilization of sugar beet carbohydrates for biological hydrogen production in the EU. Journal of Cleaner Production, 18(Supp.. 1), S9-S14. https://doi.org/10.1016/j.jclepro.2010.02.025