Propolis modulates the gut microbiota and improves the intestinal mucosal barrier function in diabetic rats

Meilan Xue, Ying Liu, Hongwei Xu, Zhitong Zhou, Yan Ma, Ting Sun, Man Liu, Huaqi Zhang, Hui Liang*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Objective: Diabetes mellitus is associated with gut microbiota disturbance and intestinal mucosal injuries. This study investigated the influence of propolis on the gut microbiota and intestinal mucosa in rats with diabetes. Methods: Sprague-Dawley (SD) rats were randomly assigned to the control group, model group, and three propolis groups (supplemented with 80, 160, and 240 mg/kg·bw propolis, respectively). A high-fat diet combined with a streptozotocin (STZ) abdominal injection were used to induce diabetes in the rats. After 4 weeks, the intestinal histopathological analysis of the ileum was observed by transmission electron microscopy. The fasting blood glucose (FBG), plasma insulin, glucose tolerance (OGTT) and glycosylated hemoglobin (HbA1c) levels were measured. The expression of tight junction (TJ) proteins in the ileum was measured using western blotting. The molecular ecology of the fecal gut microbiota was analyzed by 16S rDNA high-throughput sequencing. The contents of the short-chain fatty acids (SCFAs) in feces were measured using high-performance liquid chromatography (HPLC). Results: After propolis treatment, compared to the model group, FBG and HbA1c levels declined, while the glucose tolerance and insulin sensitivity index (ISI) increased. The levels of TJ proteins in the ileum increased in the propolis groups. The tight junctions and gap junctions of the intestinal epithelium were also improved in the propolis groups. The contents of the feces acetic acid, propionic acid and butyrate were increased in the propolis groups. 16S rDNA high-throughput sequencing revealed that the composition of the gut microbiota of rats in the propolis supplement group was significantly improved. Conclusions: Compared to the model group, propolis exerted hypoglycemic effects in diabetic rats, and it repaired intestinal mucosal damage, benefited the communities of the gut microbiota and increased SCFA levels in diabetic rats.

Original languageEnglish
Article number109393
JournalBiomedicine and Pharmacotherapy
Volume118
DOIs
Publication statusPublished - Oct 2019

Fingerprint

Propolis
Ileum
Tight Junction Proteins
Volatile Fatty Acids
Intestinal Mucosa
Ribosomal DNA
Feces
Blood Glucose
Fasting
Gastrointestinal Microbiome
Glucose
Butyrates
Tight Junctions
Gap Junctions
Glycosylated Hemoglobin A
High Fat Diet
Glucose Tolerance Test
Streptozocin
Ecology
Transmission Electron Microscopy

Keywords

  • 16S rDNA high-throughput sequencing
  • Diabetes
  • Gut microbiota
  • Propolis
  • Short chain fatty acid

Cite this

Xue, Meilan ; Liu, Ying ; Xu, Hongwei ; Zhou, Zhitong ; Ma, Yan ; Sun, Ting ; Liu, Man ; Zhang, Huaqi ; Liang, Hui. / Propolis modulates the gut microbiota and improves the intestinal mucosal barrier function in diabetic rats. In: Biomedicine and Pharmacotherapy. 2019 ; Vol. 118.
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title = "Propolis modulates the gut microbiota and improves the intestinal mucosal barrier function in diabetic rats",
abstract = "Objective: Diabetes mellitus is associated with gut microbiota disturbance and intestinal mucosal injuries. This study investigated the influence of propolis on the gut microbiota and intestinal mucosa in rats with diabetes. Methods: Sprague-Dawley (SD) rats were randomly assigned to the control group, model group, and three propolis groups (supplemented with 80, 160, and 240 mg/kg·bw propolis, respectively). A high-fat diet combined with a streptozotocin (STZ) abdominal injection were used to induce diabetes in the rats. After 4 weeks, the intestinal histopathological analysis of the ileum was observed by transmission electron microscopy. The fasting blood glucose (FBG), plasma insulin, glucose tolerance (OGTT) and glycosylated hemoglobin (HbA1c) levels were measured. The expression of tight junction (TJ) proteins in the ileum was measured using western blotting. The molecular ecology of the fecal gut microbiota was analyzed by 16S rDNA high-throughput sequencing. The contents of the short-chain fatty acids (SCFAs) in feces were measured using high-performance liquid chromatography (HPLC). Results: After propolis treatment, compared to the model group, FBG and HbA1c levels declined, while the glucose tolerance and insulin sensitivity index (ISI) increased. The levels of TJ proteins in the ileum increased in the propolis groups. The tight junctions and gap junctions of the intestinal epithelium were also improved in the propolis groups. The contents of the feces acetic acid, propionic acid and butyrate were increased in the propolis groups. 16S rDNA high-throughput sequencing revealed that the composition of the gut microbiota of rats in the propolis supplement group was significantly improved. Conclusions: Compared to the model group, propolis exerted hypoglycemic effects in diabetic rats, and it repaired intestinal mucosal damage, benefited the communities of the gut microbiota and increased SCFA levels in diabetic rats.",
keywords = "16S rDNA high-throughput sequencing, Diabetes, Gut microbiota, Propolis, Short chain fatty acid",
author = "Meilan Xue and Ying Liu and Hongwei Xu and Zhitong Zhou and Yan Ma and Ting Sun and Man Liu and Huaqi Zhang and Hui Liang",
year = "2019",
month = "10",
doi = "10.1016/j.biopha.2019.109393",
language = "English",
volume = "118",
journal = "Biomedicine and Pharmacotherapy",
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Propolis modulates the gut microbiota and improves the intestinal mucosal barrier function in diabetic rats. / Xue, Meilan; Liu, Ying; Xu, Hongwei; Zhou, Zhitong; Ma, Yan; Sun, Ting; Liu, Man; Zhang, Huaqi; Liang, Hui.

In: Biomedicine and Pharmacotherapy, Vol. 118, 109393, 10.2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Propolis modulates the gut microbiota and improves the intestinal mucosal barrier function in diabetic rats

AU - Xue, Meilan

AU - Liu, Ying

AU - Xu, Hongwei

AU - Zhou, Zhitong

AU - Ma, Yan

AU - Sun, Ting

AU - Liu, Man

AU - Zhang, Huaqi

AU - Liang, Hui

PY - 2019/10

Y1 - 2019/10

N2 - Objective: Diabetes mellitus is associated with gut microbiota disturbance and intestinal mucosal injuries. This study investigated the influence of propolis on the gut microbiota and intestinal mucosa in rats with diabetes. Methods: Sprague-Dawley (SD) rats were randomly assigned to the control group, model group, and three propolis groups (supplemented with 80, 160, and 240 mg/kg·bw propolis, respectively). A high-fat diet combined with a streptozotocin (STZ) abdominal injection were used to induce diabetes in the rats. After 4 weeks, the intestinal histopathological analysis of the ileum was observed by transmission electron microscopy. The fasting blood glucose (FBG), plasma insulin, glucose tolerance (OGTT) and glycosylated hemoglobin (HbA1c) levels were measured. The expression of tight junction (TJ) proteins in the ileum was measured using western blotting. The molecular ecology of the fecal gut microbiota was analyzed by 16S rDNA high-throughput sequencing. The contents of the short-chain fatty acids (SCFAs) in feces were measured using high-performance liquid chromatography (HPLC). Results: After propolis treatment, compared to the model group, FBG and HbA1c levels declined, while the glucose tolerance and insulin sensitivity index (ISI) increased. The levels of TJ proteins in the ileum increased in the propolis groups. The tight junctions and gap junctions of the intestinal epithelium were also improved in the propolis groups. The contents of the feces acetic acid, propionic acid and butyrate were increased in the propolis groups. 16S rDNA high-throughput sequencing revealed that the composition of the gut microbiota of rats in the propolis supplement group was significantly improved. Conclusions: Compared to the model group, propolis exerted hypoglycemic effects in diabetic rats, and it repaired intestinal mucosal damage, benefited the communities of the gut microbiota and increased SCFA levels in diabetic rats.

AB - Objective: Diabetes mellitus is associated with gut microbiota disturbance and intestinal mucosal injuries. This study investigated the influence of propolis on the gut microbiota and intestinal mucosa in rats with diabetes. Methods: Sprague-Dawley (SD) rats were randomly assigned to the control group, model group, and three propolis groups (supplemented with 80, 160, and 240 mg/kg·bw propolis, respectively). A high-fat diet combined with a streptozotocin (STZ) abdominal injection were used to induce diabetes in the rats. After 4 weeks, the intestinal histopathological analysis of the ileum was observed by transmission electron microscopy. The fasting blood glucose (FBG), plasma insulin, glucose tolerance (OGTT) and glycosylated hemoglobin (HbA1c) levels were measured. The expression of tight junction (TJ) proteins in the ileum was measured using western blotting. The molecular ecology of the fecal gut microbiota was analyzed by 16S rDNA high-throughput sequencing. The contents of the short-chain fatty acids (SCFAs) in feces were measured using high-performance liquid chromatography (HPLC). Results: After propolis treatment, compared to the model group, FBG and HbA1c levels declined, while the glucose tolerance and insulin sensitivity index (ISI) increased. The levels of TJ proteins in the ileum increased in the propolis groups. The tight junctions and gap junctions of the intestinal epithelium were also improved in the propolis groups. The contents of the feces acetic acid, propionic acid and butyrate were increased in the propolis groups. 16S rDNA high-throughput sequencing revealed that the composition of the gut microbiota of rats in the propolis supplement group was significantly improved. Conclusions: Compared to the model group, propolis exerted hypoglycemic effects in diabetic rats, and it repaired intestinal mucosal damage, benefited the communities of the gut microbiota and increased SCFA levels in diabetic rats.

KW - 16S rDNA high-throughput sequencing

KW - Diabetes

KW - Gut microbiota

KW - Propolis

KW - Short chain fatty acid

U2 - 10.1016/j.biopha.2019.109393

DO - 10.1016/j.biopha.2019.109393

M3 - Article

VL - 118

JO - Biomedicine and Pharmacotherapy

JF - Biomedicine and Pharmacotherapy

SN - 0753-3322

M1 - 109393

ER -