Propidium monoazide does not fully inhibit the detection of dead campylobacter on broiler chicken carcasses by qPCR

Ewa Pacholewicz; Arno Swart; Len J.A. Lipman; Jaap A. Wagenaar; Arie H. Havelaar; Birgitta Duim

doi:10.1016/j.mimet.2013.06.003

Propidium monoazide does not fully inhibit the detection of dead campylobacter on broiler chicken carcasses by qPCR

Ewa Pacholewicz, Arno Swart, Len J.A. Lipman, Jaap A. Wagenaar, Arie H. Havelaar, Birgitta Duim^*

^*Corresponding author for this work

Bacteriology, Host Pathogen Interaction & Diagnostics

Research output: Contribution to journal › Article › Academic › peer-review

48 Citations (Scopus)

Abstract

A real time quantitative PCR combined with propidium monoazide (PMA) treatment of samples was implemented to quantify live C. jejuni, C. coli and C. lari on broiler chicken carcasses at selected processing steps in the slaughterhouse. The samples were enumerated by culture for comparison. The Campylobacter counts determined with the PMA-qPCR and the culture method were not concordant. We conclude that the qPCR combined with PMA treatment of the samples did not fully reduce the signal from dead cells.

Original language	English
Pages (from-to)	32-38
Number of pages	7
Journal	Journal of Microbiological Methods
Volume	95
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2013.06.003
Publication status	Published - Oct 2013

Keywords

Broiler
Campylobacter
PMA
QPCR

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title = "Propidium monoazide does not fully inhibit the detection of dead campylobacter on broiler chicken carcasses by qPCR",

abstract = "A real time quantitative PCR combined with propidium monoazide (PMA) treatment of samples was implemented to quantify live C. jejuni, C. coli and C. lari on broiler chicken carcasses at selected processing steps in the slaughterhouse. The samples were enumerated by culture for comparison. The Campylobacter counts determined with the PMA-qPCR and the culture method were not concordant. We conclude that the qPCR combined with PMA treatment of the samples did not fully reduce the signal from dead cells.",

keywords = "Broiler, Campylobacter, PMA, QPCR",

author = "Ewa Pacholewicz and Arno Swart and Lipman, {Len J.A.} and Wagenaar, {Jaap A.} and Havelaar, {Arie H.} and Birgitta Duim",

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AU - Pacholewicz, Ewa

AU - Swart, Arno

AU - Lipman, Len J.A.

AU - Wagenaar, Jaap A.

AU - Havelaar, Arie H.

AU - Duim, Birgitta

PY - 2013/10

Y1 - 2013/10

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AB - A real time quantitative PCR combined with propidium monoazide (PMA) treatment of samples was implemented to quantify live C. jejuni, C. coli and C. lari on broiler chicken carcasses at selected processing steps in the slaughterhouse. The samples were enumerated by culture for comparison. The Campylobacter counts determined with the PMA-qPCR and the culture method were not concordant. We conclude that the qPCR combined with PMA treatment of the samples did not fully reduce the signal from dead cells.

KW - Broiler

KW - Campylobacter

KW - PMA

KW - QPCR

U2 - 10.1016/j.mimet.2013.06.003

DO - 10.1016/j.mimet.2013.06.003

M3 - Article

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JF - Journal of Microbiological Methods

IS - 1

ER -

Propidium monoazide does not fully inhibit the detection of dead campylobacter on broiler chicken carcasses by qPCR

Abstract

Keywords

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