Propidium monoazide does not fully inhibit the detection of dead campylobacter on broiler chicken carcasses by qPCR

Ewa Pacholewicz, Arno Swart, Len J.A. Lipman, Jaap A. Wagenaar, Arie H. Havelaar, Birgitta Duim*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

37 Citations (Scopus)

Abstract

A real time quantitative PCR combined with propidium monoazide (PMA) treatment of samples was implemented to quantify live C. jejuni, C. coli and C. lari on broiler chicken carcasses at selected processing steps in the slaughterhouse. The samples were enumerated by culture for comparison. The Campylobacter counts determined with the PMA-qPCR and the culture method were not concordant. We conclude that the qPCR combined with PMA treatment of the samples did not fully reduce the signal from dead cells.

Original languageEnglish
Pages (from-to)32-38
Number of pages7
JournalJournal of Microbiological Methods
Volume95
Issue number1
DOIs
Publication statusPublished - Oct 2013

Keywords

  • Broiler
  • Campylobacter
  • PMA
  • QPCR

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