Promoter deletions are essential for transformation of lettuce by the T-cyt gene: the phenotypes of transgenic plants

H.E.C. Curtis, W. Jordi, E. Davelaar, J.B. Power, A.M.M. de Laat, M.R. Davey

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    Abstract

    The Agrobacterium T- cyt gene was transferred into lettuce, Latuca sativa‘Saladin’ using a genotype-independent transformation procedure employing a supervirulent Agrobacterium tumefaciens strain carrying the binary vector pMOG23. Kanamycin-resistant shoots were initiated from inoculated explants only when sites were deleted within the T- cyt promoter. In culture, kanamycin-resistant shoots exhibited several phenotypes, including gall production, reduced internode length (dwarfism) and vitrification together with differences in rooting. Rooted, neomycin phosphotransferase (NPTII)-positive plants recovered from their abnormal phenotype and self pollinated to produce viable seed, following transfer to the glasshouse. Kanamycin-resistant lettuce plants exhibited increased cytokinin and chlorophyll contents compared to non-transformed plants, physiological features which could benefit long-term storage of this leafy vegetable. Copyright 1999 Annals of Botany Company
    Original languageEnglish
    Pages (from-to)559-567
    JournalAnnals of Botany
    Volume83
    Issue number5
    DOIs
    Publication statusPublished - 1999

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    Curtis, H. E. C., Jordi, W., Davelaar, E., Power, J. B., de Laat, A. M. M., & Davey, M. R. (1999). Promoter deletions are essential for transformation of lettuce by the T-cyt gene: the phenotypes of transgenic plants. Annals of Botany, 83(5), 559-567. https://doi.org/10.1006/anbo.1999.0859