Promicromonospora pachnodae sp nov., a member of the (hemi)cellulolytic hindgut flora of larvae of the scarab beetle Pachnoda marginata

A.E. Cazemier, J.C. Verdoes, F.A.G. Reubsaet, J.H.P. Hackstein, C. van der Drift, H.J.M. op den Camp

Research output: Contribution to journalArticleAcademicpeer-review

58 Citations (Scopus)

Abstract

Intestinal microorganisms play an important role in plant fiber degradation by larvae of the rose chafer Pachnoda marginata. In the hindgut of the larvae 2.5 to 7.4 × 108 bacteria per ml of gut content with xylanase or endoglucanase activity were found. Bacteria in the midgut were not (hemi)cellulolytic, but the alkaline environment in this part of the intestinal tract functions as a precellulolytic phase, solubilizing part of the lignocellulosic material. Accordingly, the degradation of lignocellulose-rich material in Pachnoda marginata larvae appeared to be a combination of a physico-chemical and microbiological process. A number of different facultative anaerobic and strictly anaerobic bacteria with (hemi)cellulolytic activity were isolated from the hindgut. A dominant (hemi)cellulolytic species was a Gram positive, irregular shaped, facultative aerobic bacterium. Further physiological identification placed the isolate in the genus Promicromonospora. Comparative 16S rDNA analysis and phenotypic features revealed that the isolate represented a new species for which the name Promicromonospora pachnodae is proposed. P. pachnodae produced xylanases and endoglucanases on several plant derived polymers, both under aerobic and anaerobic conditions.
Original languageEnglish
Pages (from-to)135-148
JournalAntonie van Leeuwenhoek: : Nederlandsch tijdschrift voor hygiëne, microbiologie en serologie
Volume83
Issue number2
DOIs
Publication statusPublished - 2003

Keywords

  • termite guts
  • cellulomonas
  • degradation
  • arthropods
  • digestion
  • bacteria
  • gene
  • microorganisms
  • lignocellulose
  • purification

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