@article{c4ecab5178a6489fa71e98f86ccb37c4,
title = "Proline-rich extensin-like receptor kinases PERK5 and PERK12 are involved in pollen tube growth",
abstract = "Proline-rich extensin-like receptor kinases (PERKs) belong to the hydroxyproline-rich glycoprotein (HRGP) superfamily known to be involved in many plant developmental processes. Here, we characterized two pollen-expressed PERKs from Arabidopsis thaliana, PERK5 and PERK12. Pollen tube growth was impaired in single and double perk5-1 perk12-1 loss of function mutants, with an impact on seed production. When the segregation was analysed, a male gametophytic defect was found, indicating that perk5-1 and perk12-1 mutants carry deficient pollen transmission. Furthermore, perk5-1 perk12-1 displayed an excessive accumulation of pectins and cellulose at the cell wall of the pollen tubes. Our results indicate that PERK5 and PERK12 are necessary for proper pollen tube growth, highlighting their role in cell wall assembly and reactive oxygen species homeostasis.",
keywords = "Arabidopsis thaliana, cell wall, PERKs, polar growth, pollen tubes",
author = "Cecilia Borassi and Sede, {Ana R.} and Mecchia, {Mart{\'i}n A.} and Silvina Mangano and Eliana Marzol and Denita-Juarez, {Silvina P.} and {Salgado Salter}, {Juan D.} and Velasquez, {Silvia M.} and Muschietti, {Jorge P.} and Estevez, {Jos{\'e} M.}",
note = "Funding Information: We would like to acknowledge L. Ca?rdenas for providing LAT52::HyPer sensor line. We thank ABRC (Ohio State University) for providing T-DNA seed lines. JME and JPM are investigators of the National Research Council (CONICET) from Argentina. This work was supported by a grants from the Agencia Nacional de Promocio?n Cienti?fica y Tecnolo?gica (ANPCyT) (PICT2017-0066, PICT2019-0015), ANID ? Programa Iniciativa Cienti?fica Milenio ICN17_022 and Fondo Nacional de Desarrollo Cienti?fico y Tecnolo?gico [1200010] to JME and from the ANPCyT (PICT2017-0076, PICT2018-0504) to JPM The authors declare that they have no competing financial interests. Correspondence and requests for materials should be addressed to JPM or JME. Funding Information: We would like to acknowledge L. C{\'a}rdenas for providing LAT52::HyPer sensor line. We thank ABRC (Ohio State University) for providing T‐DNA seed lines. JME and JPM are investigators of the National Research Council (CONICET) from Argentina. This work was supported by a grants from the Agencia Nacional de Promoci{\'o}n Cient{\'i}fica y Tecnol{\'o}gica (ANPCyT) (PICT2017‐0066, PICT2019‐0015), ANID – Programa Iniciativa Cient{\'i}fica Milenio ICN17_022 and Fondo Nacional de Desarrollo Cient{\'i}fico y Tecnol{\'o}gico [1200010] to JME and from the ANPCyT (PICT2017‐0076, PICT2018‐0504) to JPM The authors declare that they have no competing financial interests. Correspondence and requests for materials should be addressed to JPM or JME. Publisher Copyright: {\textcopyright} 2021 Federation of European Biochemical Societies",
year = "2021",
month = oct,
doi = "10.1002/1873-3468.14185",
language = "English",
volume = "595",
pages = "2593--2607",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "20",
}