Proline-rich extensin-like receptor kinases PERK5 and PERK12 are involved in pollen tube growth

Cecilia Borassi, Ana R. Sede, Martín A. Mecchia, Silvina Mangano, Eliana Marzol, Silvina P. Denita-Juarez, Juan D. Salgado Salter, Silvia M. Velasquez, Jorge P. Muschietti, José M. Estevez*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

Proline-rich extensin-like receptor kinases (PERKs) belong to the hydroxyproline-rich glycoprotein (HRGP) superfamily known to be involved in many plant developmental processes. Here, we characterized two pollen-expressed PERKs from Arabidopsis thaliana, PERK5 and PERK12. Pollen tube growth was impaired in single and double perk5-1 perk12-1 loss of function mutants, with an impact on seed production. When the segregation was analysed, a male gametophytic defect was found, indicating that perk5-1 and perk12-1 mutants carry deficient pollen transmission. Furthermore, perk5-1 perk12-1 displayed an excessive accumulation of pectins and cellulose at the cell wall of the pollen tubes. Our results indicate that PERK5 and PERK12 are necessary for proper pollen tube growth, highlighting their role in cell wall assembly and reactive oxygen species homeostasis.

Original languageEnglish
Pages (from-to)2593-2607
Number of pages15
JournalFEBS Letters
Volume595
Issue number20
DOIs
Publication statusPublished - Oct 2021
Externally publishedYes

Keywords

  • Arabidopsis thaliana
  • cell wall
  • PERKs
  • polar growth
  • pollen tubes

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