Prokaryotic argonaute proteins and uses thereof

J. van der Oost (Inventor), J.W. Hegge (Inventor)

Research output: PatentOther research output

Abstract

The invention relates to the field of genetic engineering tools, methods and techniques for nucleic acid, gene or genome editing. Specifically, the invention concerns prokaryotic Argonaute (pAgo) polypeptides having nuclease activity against target DNA when pAgo is complexed with a DNA guide. The invention also provides expression vectors comprising nucleic acids encoding said polypeptides as well as compositions and kits for, and methods of cleaving and editing target nucleic acids in a sequence-specific manner. The polypeptides, nucleic acids, expression vectors, compositions, kits and methods of the invention allow site- specific modifications of genetic material, whether isolated from cells in vitro, or within cells in situ and as such may usefully find application in many fields of biotechnology, including, for example, synthetic biology, gene therapy and agricultural or microbial biotechnology.
Original languageEnglish
Patent numberWO2018011236
Priority date7/12/16
Publication statusPublished - 18 Jan 2018

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Argonaute Proteins
Nucleic Acids
Biotechnology
Peptides
Synthetic Biology
Synthetic Genes
Genetic Techniques
Genetic Engineering
DNA
Genetic Therapy
Genes

Cite this

van der Oost, J., & Hegge, J. W. (2018). Prokaryotic argonaute proteins and uses thereof. (Patent No. WO2018011236).
van der Oost, J. (Inventor) ; Hegge, J.W. (Inventor). / Prokaryotic argonaute proteins and uses thereof. Patent No.: WO2018011236.
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van der Oost, J & Hegge, JW 2018, Prokaryotic argonaute proteins and uses thereof, Patent No. WO2018011236.

Prokaryotic argonaute proteins and uses thereof. / van der Oost, J. (Inventor); Hegge, J.W. (Inventor).

Patent No.: WO2018011236.

Research output: PatentOther research output

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T1 - Prokaryotic argonaute proteins and uses thereof

AU - van der Oost, J.

AU - Hegge, J.W.

PY - 2018/1/18

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N2 - The invention relates to the field of genetic engineering tools, methods and techniques for nucleic acid, gene or genome editing. Specifically, the invention concerns prokaryotic Argonaute (pAgo) polypeptides having nuclease activity against target DNA when pAgo is complexed with a DNA guide. The invention also provides expression vectors comprising nucleic acids encoding said polypeptides as well as compositions and kits for, and methods of cleaving and editing target nucleic acids in a sequence-specific manner. The polypeptides, nucleic acids, expression vectors, compositions, kits and methods of the invention allow site- specific modifications of genetic material, whether isolated from cells in vitro, or within cells in situ and as such may usefully find application in many fields of biotechnology, including, for example, synthetic biology, gene therapy and agricultural or microbial biotechnology.

AB - The invention relates to the field of genetic engineering tools, methods and techniques for nucleic acid, gene or genome editing. Specifically, the invention concerns prokaryotic Argonaute (pAgo) polypeptides having nuclease activity against target DNA when pAgo is complexed with a DNA guide. The invention also provides expression vectors comprising nucleic acids encoding said polypeptides as well as compositions and kits for, and methods of cleaving and editing target nucleic acids in a sequence-specific manner. The polypeptides, nucleic acids, expression vectors, compositions, kits and methods of the invention allow site- specific modifications of genetic material, whether isolated from cells in vitro, or within cells in situ and as such may usefully find application in many fields of biotechnology, including, for example, synthetic biology, gene therapy and agricultural or microbial biotechnology.

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van der Oost J, Hegge JW, inventors. Prokaryotic argonaute proteins and uses thereof. WO2018011236. 2018 Jan 18.