Progressive bovine paratuberculosis is associated with local loss of CD4(+) T cells, increased frequency of gamma delta T cells, and related changes in T-cell function

A. Koets, V. Rutten, A. van Hoek, F. van Mil, K. Muller, D. Bakker, E. Gruys, W. van Eden

Research output: Contribution to journalArticleAcademicpeer-review

81 Citations (Scopus)

Abstract

Bovine paratuberculosis is caused by the infection of young calves with Mycobacterium avium subsp. paratuberculosis, resulting in a chronic granulomatous infection of predominantly the ileum. After an incubation period of 2 to 5 years, the disease becomes progressive in some of the chronically infected, but asymptomatic cows. This results in a protein-losing enteropathy that will ultimately be fatal. A loss of cell-mediated immune responses in symptomatic animals has been described, but no information is available concerning immune reactivity in the intestine. We sought to investigate putative disease status-associated lymphocyte subset distributions and antigen-specific functional characteristics of mononuclear cells isolated from blood, gut-associated lymphoid tissue, and the intestinal walls of 22 cows in different stages of disease and in control animals. The results demonstrated a significant decrease in CD4+ T-cell frequency and a significant increase in TcR1-N12+ T-cell frequency in ileum lamina propria lymphocytes of symptomatic animals compared to the asymptomatic shedders. Immunohistology revealed that there was also an absolute decrease in the number of CD4+ T cells in sections of the lesional ileum. Our findings also indicated that both peripheral and intestinal cell-mediated responses are decreased in symptomatic animals compared to asymptomatic animals. We conclude that the decrease in cell-mediated responses is likely related to a loss of antigen-specific CD4+ T cells, which is most prominent in the lesional ileum from symptomatic animals, thus contributing to the progressive nature of bovine paratuberculosis
Original languageEnglish
Pages (from-to)3856-3864
JournalInfection and Immunity
Volume70
Issue number7
DOIs
Publication statusPublished - 2002

Keywords

  • blood mononuclear-cells
  • lymphoblast proliferative capacity
  • johnes-disease
  • monoclonal-antibodies
  • mycobacterium-paratuberculosis
  • para-tuberculosis
  • lymphocytes-t
  • ovine paratuberculosis
  • lymph-node
  • alpha-beta

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