Abstract
Adipose tissue is an endocrine organ capable of secreting a number of adipokines with a role in the regulation of adipose tissue and whole-body metabolism. We used two-dimensional gel electrophoresis combined with mass spectrometry to profile the secreted proteins from (pre)adipocytes. The culture medium of 3T3-L1 cells during adipocyte differentiation was screened, and 41 proteins that responded to blocking of secretion by 20°C treatment and/or brefeldin A treatment were identified. Prohibitin, stress-70 protein, and adhesion-regulating molecule 1 are reported for the first time as secreted proteins. In addition, procollagen C-proteinase enhancer protein, galectin-1, cyclophilin A and C, and SF20/IL-25 are newly identified as adipocyte secreted factors. Secretion profiles indicated a dynamic environment including an actively remodeling extracellular matrix and several factors involved in growth regulation
| Original language | English |
|---|---|
| Pages (from-to) | 2405-2417 |
| Journal | Cellular and Molecular Life Sciences |
| Volume | 61 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2004 |
Keywords
- inhibitory factor mif
- cis-trans-isomerases
- adipose-tissue
- extracellular-matrix
- gene-expression
- potential role
- insulin-resistance
- membrane-proteins
- alpha-enolase
- growth-factor