Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines

P. Wang, E. Mariman, J. Keijer, J.P. Noben, J. Robben, J. Renes

    Research output: Contribution to journalArticleAcademicpeer-review

    121 Citations (Scopus)

    Abstract

    Adipose tissue is an endocrine organ capable of secreting a number of adipokines with a role in the regulation of adipose tissue and whole-body metabolism. We used two-dimensional gel electrophoresis combined with mass spectrometry to profile the secreted proteins from (pre)adipocytes. The culture medium of 3T3-L1 cells during adipocyte differentiation was screened, and 41 proteins that responded to blocking of secretion by 20°C treatment and/or brefeldin A treatment were identified. Prohibitin, stress-70 protein, and adhesion-regulating molecule 1 are reported for the first time as secreted proteins. In addition, procollagen C-proteinase enhancer protein, galectin-1, cyclophilin A and C, and SF20/IL-25 are newly identified as adipocyte secreted factors. Secretion profiles indicated a dynamic environment including an actively remodeling extracellular matrix and several factors involved in growth regulation
    Original languageEnglish
    Pages (from-to)2405-2417
    JournalCellular and Molecular Life Sciences
    Volume61
    Issue number8
    DOIs
    Publication statusPublished - 2004

    Keywords

    • inhibitory factor mif
    • cis-trans-isomerases
    • adipose-tissue
    • extracellular-matrix
    • gene-expression
    • potential role
    • insulin-resistance
    • membrane-proteins
    • alpha-enolase
    • growth-factor

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