White spot syndrome virus (WSSV) is a major shrimp pathogen and it has been reported that short-lived protection in shrimp, against this virus, can be provided using ‘vaccines’ containing WSSV envelope protein VP28 or formalin-inactivated WSSV. Nevertheless, the mechanisms of protection through these vaccines are not well described. Therefore, the interactions with the shrimp immune system need to be ascertained, in order to improve immunogenicity of the vaccines. In the present study, the aforementioned candidate vaccines were orally intubated in P. monodon and the expression profiles of proteins in the gut were determined by employing two-dimensional gel electrophoresis (2-DE). The same technique was also used on shrimps infected orally with live-WSSV to identify the differences in protein profiles. Around 90 proteins spots, with an altered expression (at least 1.5 fold), were subjected to mass spectrometry, and further analysed using bioinformatic databases. Based on the biological Gene Ontology terms, these proteins were classified under energy production, phenol oxidase activity, apoptosis, serine proteases, intracellular transport or nucleic acid synthesis. Additionally, spot intensities of some immune relevant proteins were compared with their corresponding mRNA levels (determined by quantitative real-time PCR). The results identified proteins that could be linked to the shrimp's immune response to vaccines.
|Journal||Fish and Shellfish Immunology|
|Publication status||Published - 2013|