Production, Refining, Structural Characterization and Fermentability of Rice Husk Xylooligosaccharides

P. Gullon, M.J. Gonzales-Munoz, M.P. van Gool, H.A. Schols, J. Hirsch, A. Ebringerova, J.C. Rarajo

Research output: Contribution to journalArticleAcademicpeer-review

63 Citations (Scopus)

Abstract

Oligosaccharides produced by hydrothermal processing of rice husks (xylooligosaccharides and glucooligosaccharides) were refined by membrane processing (operating in diafiltration and concentration modes), subjected to xylanase treatment to reduce the average molar mass, and subjected to further purification by ultrafiltration (operating in concentration mode) and ion exchange. The purified products were assayed for composition, molar mass distribution and structural characterization by HPLC, HPAEC-PAD, HPSEC, MALDI-TOF-MS and NMR (1H and 13C). The fermentability of the purified product by fecal inocula was assessed on the basis of the time courses of pH and oligosaccharide concentrations. Succinate, lactate, formiate, acetate, propionate and butyrate were the major products resulting from fermentation experiments.
Original languageEnglish
Pages (from-to)3632-3641
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number6
DOIs
Publication statusPublished - 2010

Keywords

  • substituted xylo-oligosaccharides
  • human colonic microbiota
  • treated eucalyptus wood
  • in-vitro
  • dietary modulation
  • ft-ir
  • autohydrolysis
  • fermentation
  • liquors
  • polysaccharides

Fingerprint Dive into the research topics of 'Production, Refining, Structural Characterization and Fermentability of Rice Husk Xylooligosaccharides'. Together they form a unique fingerprint.

Cite this