Production, Refining, Structural Characterization and Fermentability of Rice Husk Xylooligosaccharides

P. Gullon; M.J. Gonzales-Munoz; M.P. van Gool; H.A. Schols; J. Hirsch; A. Ebringerova; J.C. Rarajo

doi:10.1021/jf904508g

Production, Refining, Structural Characterization and Fermentability of Rice Husk Xylooligosaccharides

P. Gullon, M.J. Gonzales-Munoz, M.P. van Gool, H.A. Schols, J. Hirsch, A. Ebringerova, J.C. Rarajo

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Oligosaccharides produced by hydrothermal processing of rice husks (xylooligosaccharides and glucooligosaccharides) were refined by membrane processing (operating in diafiltration and concentration modes), subjected to xylanase treatment to reduce the average molar mass, and subjected to further purification by ultrafiltration (operating in concentration mode) and ion exchange. The purified products were assayed for composition, molar mass distribution and structural characterization by HPLC, HPAEC-PAD, HPSEC, MALDI-TOF-MS and NMR (1H and 13C). The fermentability of the purified product by fecal inocula was assessed on the basis of the time courses of pH and oligosaccharide concentrations. Succinate, lactate, formiate, acetate, propionate and butyrate were the major products resulting from fermentation experiments.

Original language	English
Pages (from-to)	3632-3641
Journal	Journal of Agricultural and Food Chemistry
Volume	58
Issue number	6
DOIs	https://doi.org/10.1021/jf904508g
Publication status	Published - 2010

Keywords

substituted xylo-oligosaccharides
human colonic microbiota
treated eucalyptus wood
in-vitro
dietary modulation
ft-ir
autohydrolysis
fermentation
liquors
polysaccharides

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10.1021/jf904508g

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title = "Production, Refining, Structural Characterization and Fermentability of Rice Husk Xylooligosaccharides",

abstract = "Oligosaccharides produced by hydrothermal processing of rice husks (xylooligosaccharides and glucooligosaccharides) were refined by membrane processing (operating in diafiltration and concentration modes), subjected to xylanase treatment to reduce the average molar mass, and subjected to further purification by ultrafiltration (operating in concentration mode) and ion exchange. The purified products were assayed for composition, molar mass distribution and structural characterization by HPLC, HPAEC-PAD, HPSEC, MALDI-TOF-MS and NMR (1H and 13C). The fermentability of the purified product by fecal inocula was assessed on the basis of the time courses of pH and oligosaccharide concentrations. Succinate, lactate, formiate, acetate, propionate and butyrate were the major products resulting from fermentation experiments.",

keywords = "substituted xylo-oligosaccharides, human colonic microbiota, treated eucalyptus wood, in-vitro, dietary modulation, ft-ir, autohydrolysis, fermentation, liquors, polysaccharides",

author = "P. Gullon and M.J. Gonzales-Munoz and {van Gool}, M.P. and H.A. Schols and J. Hirsch and A. Ebringerova and J.C. Rarajo",

year = "2010",

doi = "10.1021/jf904508g",

language = "English",

volume = "58",

pages = "3632--3641",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

number = "6",

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TY - JOUR

T1 - Production, Refining, Structural Characterization and Fermentability of Rice Husk Xylooligosaccharides

AU - Gullon, P.

AU - Gonzales-Munoz, M.J.

AU - van Gool, M.P.

AU - Schols, H.A.

AU - Hirsch, J.

AU - Ebringerova, A.

AU - Rarajo, J.C.

PY - 2010

Y1 - 2010

N2 - Oligosaccharides produced by hydrothermal processing of rice husks (xylooligosaccharides and glucooligosaccharides) were refined by membrane processing (operating in diafiltration and concentration modes), subjected to xylanase treatment to reduce the average molar mass, and subjected to further purification by ultrafiltration (operating in concentration mode) and ion exchange. The purified products were assayed for composition, molar mass distribution and structural characterization by HPLC, HPAEC-PAD, HPSEC, MALDI-TOF-MS and NMR (1H and 13C). The fermentability of the purified product by fecal inocula was assessed on the basis of the time courses of pH and oligosaccharide concentrations. Succinate, lactate, formiate, acetate, propionate and butyrate were the major products resulting from fermentation experiments.

AB - Oligosaccharides produced by hydrothermal processing of rice husks (xylooligosaccharides and glucooligosaccharides) were refined by membrane processing (operating in diafiltration and concentration modes), subjected to xylanase treatment to reduce the average molar mass, and subjected to further purification by ultrafiltration (operating in concentration mode) and ion exchange. The purified products were assayed for composition, molar mass distribution and structural characterization by HPLC, HPAEC-PAD, HPSEC, MALDI-TOF-MS and NMR (1H and 13C). The fermentability of the purified product by fecal inocula was assessed on the basis of the time courses of pH and oligosaccharide concentrations. Succinate, lactate, formiate, acetate, propionate and butyrate were the major products resulting from fermentation experiments.

KW - substituted xylo-oligosaccharides

KW - human colonic microbiota

KW - treated eucalyptus wood

KW - in-vitro

KW - dietary modulation

KW - ft-ir

KW - autohydrolysis

KW - fermentation

KW - liquors

KW - polysaccharides

U2 - 10.1021/jf904508g

DO - 10.1021/jf904508g

M3 - Article

SN - 0021-8561

VL - 58

SP - 3632

EP - 3641

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 6

ER -

Production, Refining, Structural Characterization and Fermentability of Rice Husk Xylooligosaccharides

Abstract

Keywords

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