Production of (+)-valencene in the mushroom-forming fungus S. commune

K. Scholtmeijer, J. Beekwilder, K. Cankar, H.A.B. Wösten, L.G. Lugones, H.J. Bosch

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

Production of commercially interesting sesquiterpenes was previously examined in plants and microorganisms such as Escherichia coli and Saccharomyces cerevisiae. We here investigate the potential of the mushroom Schizophyllum commune for the production of sesquiterpenes. Genomic analysis of S. commune revealed that the mevalonate pathway required for the synthesis of the farnesyl diphosphate substrate for sesquiterpene production is operational. Introduction of a valencene synthase gene resulted in production of the sesquiterpene (+)-valencene, both in mycelium and in fruiting bodies. Levels of (+)-valencene in culture media of strains containing a mutated RGS regulatory protein gene (thn) were increased fourfold compared to those in wild-type transformants. Up to 16 mg L-1 (+)-valencene was produced in these strains. In addition, the amount of (+)-valencene containing n-dodecane recovered from the culture medium increased sixfold to sevenfold in the thn mutant strains due to the absence of schizophyllan.
LanguageEnglish
Pages5059-5068
JournalApplied Microbiology and Biotechnology
Volume98
Issue number11
DOIs
Publication statusPublished - 2014

Fingerprint

Agaricales
Sesquiterpenes
Fungi
Schizophyllum
Culture Media
Sizofiran
RGS Proteins
Mevalonic Acid
Mycelium
Regulator Genes
Saccharomyces cerevisiae
valencene
Escherichia coli
Genes

Keywords

  • basidiomycete schizophyllum-commune
  • sc3 hydrophobin gene
  • secondary metabolism
  • filamentous fungi
  • aerial hyphae
  • biosynthesis
  • mutation
  • protein
  • sesquiterpenes
  • microorganisms

Cite this

@article{946973b9b08d4ec0ac13b32a26f536f9,
title = "Production of (+)-valencene in the mushroom-forming fungus S. commune",
abstract = "Production of commercially interesting sesquiterpenes was previously examined in plants and microorganisms such as Escherichia coli and Saccharomyces cerevisiae. We here investigate the potential of the mushroom Schizophyllum commune for the production of sesquiterpenes. Genomic analysis of S. commune revealed that the mevalonate pathway required for the synthesis of the farnesyl diphosphate substrate for sesquiterpene production is operational. Introduction of a valencene synthase gene resulted in production of the sesquiterpene (+)-valencene, both in mycelium and in fruiting bodies. Levels of (+)-valencene in culture media of strains containing a mutated RGS regulatory protein gene (thn) were increased fourfold compared to those in wild-type transformants. Up to 16 mg L-1 (+)-valencene was produced in these strains. In addition, the amount of (+)-valencene containing n-dodecane recovered from the culture medium increased sixfold to sevenfold in the thn mutant strains due to the absence of schizophyllan.",
keywords = "basidiomycete schizophyllum-commune, sc3 hydrophobin gene, secondary metabolism, filamentous fungi, aerial hyphae, biosynthesis, mutation, protein, sesquiterpenes, microorganisms",
author = "K. Scholtmeijer and J. Beekwilder and K. Cankar and H.A.B. W{\"o}sten and L.G. Lugones and H.J. Bosch",
year = "2014",
doi = "10.1007/s00253-014-5581-2",
language = "English",
volume = "98",
pages = "5059--5068",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "11",

}

Production of (+)-valencene in the mushroom-forming fungus S. commune. / Scholtmeijer, K.; Beekwilder, J.; Cankar, K.; Wösten, H.A.B.; Lugones, L.G.; Bosch, H.J.

In: Applied Microbiology and Biotechnology, Vol. 98, No. 11, 2014, p. 5059-5068.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Production of (+)-valencene in the mushroom-forming fungus S. commune

AU - Scholtmeijer, K.

AU - Beekwilder, J.

AU - Cankar, K.

AU - Wösten, H.A.B.

AU - Lugones, L.G.

AU - Bosch, H.J.

PY - 2014

Y1 - 2014

N2 - Production of commercially interesting sesquiterpenes was previously examined in plants and microorganisms such as Escherichia coli and Saccharomyces cerevisiae. We here investigate the potential of the mushroom Schizophyllum commune for the production of sesquiterpenes. Genomic analysis of S. commune revealed that the mevalonate pathway required for the synthesis of the farnesyl diphosphate substrate for sesquiterpene production is operational. Introduction of a valencene synthase gene resulted in production of the sesquiterpene (+)-valencene, both in mycelium and in fruiting bodies. Levels of (+)-valencene in culture media of strains containing a mutated RGS regulatory protein gene (thn) were increased fourfold compared to those in wild-type transformants. Up to 16 mg L-1 (+)-valencene was produced in these strains. In addition, the amount of (+)-valencene containing n-dodecane recovered from the culture medium increased sixfold to sevenfold in the thn mutant strains due to the absence of schizophyllan.

AB - Production of commercially interesting sesquiterpenes was previously examined in plants and microorganisms such as Escherichia coli and Saccharomyces cerevisiae. We here investigate the potential of the mushroom Schizophyllum commune for the production of sesquiterpenes. Genomic analysis of S. commune revealed that the mevalonate pathway required for the synthesis of the farnesyl diphosphate substrate for sesquiterpene production is operational. Introduction of a valencene synthase gene resulted in production of the sesquiterpene (+)-valencene, both in mycelium and in fruiting bodies. Levels of (+)-valencene in culture media of strains containing a mutated RGS regulatory protein gene (thn) were increased fourfold compared to those in wild-type transformants. Up to 16 mg L-1 (+)-valencene was produced in these strains. In addition, the amount of (+)-valencene containing n-dodecane recovered from the culture medium increased sixfold to sevenfold in the thn mutant strains due to the absence of schizophyllan.

KW - basidiomycete schizophyllum-commune

KW - sc3 hydrophobin gene

KW - secondary metabolism

KW - filamentous fungi

KW - aerial hyphae

KW - biosynthesis

KW - mutation

KW - protein

KW - sesquiterpenes

KW - microorganisms

U2 - 10.1007/s00253-014-5581-2

DO - 10.1007/s00253-014-5581-2

M3 - Article

VL - 98

SP - 5059

EP - 5068

JO - Applied Microbiology and Biotechnology

T2 - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 11

ER -