Production of resveratrol in recombinant microorganisms

M.J. Beekwilder, R. Wolswinkel, H.H. Jonker, R.D. Hall, C.H. de Vos, A.G. Bovy

    Research output: Contribution to journalArticleAcademicpeer-review

    140 Citations (Scopus)

    Abstract

    Resveratrol production in Saccharomyces cerevisiae was compared to that in Escherichia coli. In both systems, 4-coumarate:coenzyme A ligase from tobacco and stilbene synthase from grapes were expressed. When p-coumaric acid was used as the precursor, resveratrol accumulations in the culture medium were observed to be comparable in E. coli (16 mg/liter) and yeast (6 mg/liter)
    Original languageEnglish
    Pages (from-to)5670-5672
    JournalApplied and Environmental Microbiology
    Volume72
    Issue number8
    DOIs
    Publication statusPublished - 2006

    Fingerprint

    resveratrol
    tobacco
    yeast
    microorganism
    Coenzyme A Ligases
    Escherichia coli
    microorganisms
    coenzyme A
    acid
    p-coumaric acid
    Vitis
    ligases
    Tobacco
    Saccharomyces cerevisiae
    Culture Media
    grapes
    culture media
    Yeasts
    yeasts
    trihydroxystilbene synthase

    Keywords

    • engineered escherichia-coli
    • saccharomyces-cerevisiae
    • biosynthesis
    • pathway

    Cite this

    Beekwilder, M.J. ; Wolswinkel, R. ; Jonker, H.H. ; Hall, R.D. ; de Vos, C.H. ; Bovy, A.G. / Production of resveratrol in recombinant microorganisms. In: Applied and Environmental Microbiology. 2006 ; Vol. 72, No. 8. pp. 5670-5672.
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    author = "M.J. Beekwilder and R. Wolswinkel and H.H. Jonker and R.D. Hall and {de Vos}, C.H. and A.G. Bovy",
    year = "2006",
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    Production of resveratrol in recombinant microorganisms. / Beekwilder, M.J.; Wolswinkel, R.; Jonker, H.H.; Hall, R.D.; de Vos, C.H.; Bovy, A.G.

    In: Applied and Environmental Microbiology, Vol. 72, No. 8, 2006, p. 5670-5672.

    Research output: Contribution to journalArticleAcademicpeer-review

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    T1 - Production of resveratrol in recombinant microorganisms

    AU - Beekwilder, M.J.

    AU - Wolswinkel, R.

    AU - Jonker, H.H.

    AU - Hall, R.D.

    AU - de Vos, C.H.

    AU - Bovy, A.G.

    PY - 2006

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    N2 - Resveratrol production in Saccharomyces cerevisiae was compared to that in Escherichia coli. In both systems, 4-coumarate:coenzyme A ligase from tobacco and stilbene synthase from grapes were expressed. When p-coumaric acid was used as the precursor, resveratrol accumulations in the culture medium were observed to be comparable in E. coli (16 mg/liter) and yeast (6 mg/liter)

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    KW - engineered escherichia-coli

    KW - saccharomyces-cerevisiae

    KW - biosynthesis

    KW - pathway

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    DO - 10.1128/AEM.00609-06

    M3 - Article

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    JF - Applied and Environmental Microbiology

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