Production of polyhedral inclusion bodies from Helicoverpa armigera larvae infected with wild-type and recombinant HaSNPV

X. Sun, X.C. Sun, B. Bai, W. van der Werf, J.M. Vlak, Z. Hu

Research output: Contribution to journalArticleAcademicpeer-review

24 Citations (Scopus)

Abstract

We report on the yield of Polyhedral Inclusion Bodies (PIBs) from first to fifth instar Helicoverpa armigera larvae inoculated with wild-type Helicoverpa armigera nucleopolyhedrovirus (HaSNPV-WT) or with one of two HaSNPV recombinants, one in which the ecdysteroid UDP-glucosyltransferase (egt) gene was deleted (HaSNPV-EGTD) and a second in which the egt gene was replaced by a scorpion toxin (AaIT) gene (HaSNPV-AaIT). A significant linear relationship between the logarithm of cadaver weight and the logarithm of the number of PIBs per cadaver was observed for all three virus types. The increase of the number of PIBs with larval weight was significantly greater for HaSNPV-WT than for the recombinant viruses. For each of the three HaSNPVs, PIB yield per cadaver was significantly affected by larval instar at death and by time to death, with later instars and longer surviving larvae producing a greater number of PIBs. As both recombinants caused host larvae to die at earlier instars than HaSNPV-WT, their virus yields were significantly reduced. Virus yield per larva, inoculated with HaSNPV-AaIT in the first, second, third, fourth or fifth larval stage was 23, 32, 41, 44 and 47% of the yield of HaSNPV-WT, respectively. For HaSNPV-EGTD, virus yield per larva inoculated in first through fifth instar, respectively, was 41, 55, 63, 54 and 82% of the yield of HaSNPV-WT. These results provide a basis for optimizing the production regime of recombinant HaSNPVs in larvae and for modeling the behavior of these viruses in agro-ecosystems
Original languageEnglish
Pages (from-to)353-366
JournalBiocontrol Science and Technology
Volume15
Issue number4
DOIs
Publication statusPublished - 2005

Keywords

  • single-nucleocapsid nucleopolyhedrovirus
  • trichoplusia ni larvae
  • process-based model
  • autographa-californica
  • biological-control
  • spodoptera-exigua
  • beet armyworm
  • baculoviruses
  • heliothis
  • greenhouses

Fingerprint Dive into the research topics of 'Production of polyhedral inclusion bodies from Helicoverpa armigera larvae infected with wild-type and recombinant HaSNPV'. Together they form a unique fingerprint.

Cite this