It has been found that esterification of a hydroxy-fatty acid by a lipase can be coupled with oleate hydratase (OHase) generation of that hydroxy-FA from an unsaturated FA with a cis C9-C10 double bond, e.g. oleic acid, in a single aqueous buffered reaction medium at low temperature,e.g. 30° C. A simple one-pot enzymatic method to produce fatty acid estolides from one or more triglycerides, e.g. starting from a natural plant oil, is thereby enabled in which the same lipase catalyses both the initial hydrolysis of triglyceride and the final esterification step.
|Publication status||Published - 29 Sep 2016|
Boeriu, C. G., Todea, A., Arends, I. W. C. E., & Otten, L. G. (2016). Production of fatty acid estolides. (Patent No. WO2016151115). https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2016151115A1&KC=A1&FT=D&ND=4&date=20160929&DB=&locale=en_EP#