Production methods for heparosan, a precursor of heparin and heparan sulfate

A.A.E. Chavaroche, L.A.M. van den Broek, G. Eggink

Research output: Contribution to journalArticleAcademicpeer-review

12 Citations (Scopus)

Abstract

Heparin and heparan sulfate belong to the glycosaminoglycan family. Heparin which is known as a powerful anticoagulant has been also described to have potential in therapeutic applications such as in the treatment against cancer and prevention of virus infections. Heparan sulfate, an analog of heparin, which is not used for medical purposes yet, was reported to have the same pharmaceutical potential as heparin. Both heparin and heparan sulfate share a common precursor molecule known as heparosan. Heparosan determines the polymer chain length and the sugar unit backbone composition, which are determinant structural parameters for the biological activity of heparin and heparan sulfate. In this review we give an overview of the different methods used to synthesize heparosan, and we highlight the pro and cons of each method in respect to the synthesis of bioengineered heparin-like molecules. Advancements in the field of the synthesis of bioengineered heparin are also reported.
Original languageEnglish
Pages (from-to)38-47
JournalCarbohydrate Polymers
Volume93
Issue number1
DOIs
Publication statusPublished - 2013

Fingerprint

Heparitin Sulfate
Heparin
Molecules
Oncology
heparosan
Sulfates
Bioactivity
Glycosaminoglycans
Chain length
Viruses
Sugars
Drug products
Anticoagulants
Polymers
Chemical analysis
Pharmaceutical Preparations

Keywords

  • n-deacetylase/n-sulfotransferase
  • k5 capsular polysaccharide
  • multocida type-a
  • escherichia-coli
  • pasteurella-multocida
  • chemoenzymatic synthesis
  • molecular-weight
  • hyaluronic-acid
  • sulfate/heparin biosynthesis
  • bioengineered heparin

Cite this

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title = "Production methods for heparosan, a precursor of heparin and heparan sulfate",
abstract = "Heparin and heparan sulfate belong to the glycosaminoglycan family. Heparin which is known as a powerful anticoagulant has been also described to have potential in therapeutic applications such as in the treatment against cancer and prevention of virus infections. Heparan sulfate, an analog of heparin, which is not used for medical purposes yet, was reported to have the same pharmaceutical potential as heparin. Both heparin and heparan sulfate share a common precursor molecule known as heparosan. Heparosan determines the polymer chain length and the sugar unit backbone composition, which are determinant structural parameters for the biological activity of heparin and heparan sulfate. In this review we give an overview of the different methods used to synthesize heparosan, and we highlight the pro and cons of each method in respect to the synthesis of bioengineered heparin-like molecules. Advancements in the field of the synthesis of bioengineered heparin are also reported.",
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author = "A.A.E. Chavaroche and {van den Broek}, L.A.M. and G. Eggink",
year = "2013",
doi = "10.1016/j.carbpol.2012.04.046",
language = "English",
volume = "93",
pages = "38--47",
journal = "Carbohydrate Polymers",
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Production methods for heparosan, a precursor of heparin and heparan sulfate. / Chavaroche, A.A.E.; van den Broek, L.A.M.; Eggink, G.

In: Carbohydrate Polymers, Vol. 93, No. 1, 2013, p. 38-47.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Production methods for heparosan, a precursor of heparin and heparan sulfate

AU - Chavaroche, A.A.E.

AU - van den Broek, L.A.M.

AU - Eggink, G.

PY - 2013

Y1 - 2013

N2 - Heparin and heparan sulfate belong to the glycosaminoglycan family. Heparin which is known as a powerful anticoagulant has been also described to have potential in therapeutic applications such as in the treatment against cancer and prevention of virus infections. Heparan sulfate, an analog of heparin, which is not used for medical purposes yet, was reported to have the same pharmaceutical potential as heparin. Both heparin and heparan sulfate share a common precursor molecule known as heparosan. Heparosan determines the polymer chain length and the sugar unit backbone composition, which are determinant structural parameters for the biological activity of heparin and heparan sulfate. In this review we give an overview of the different methods used to synthesize heparosan, and we highlight the pro and cons of each method in respect to the synthesis of bioengineered heparin-like molecules. Advancements in the field of the synthesis of bioengineered heparin are also reported.

AB - Heparin and heparan sulfate belong to the glycosaminoglycan family. Heparin which is known as a powerful anticoagulant has been also described to have potential in therapeutic applications such as in the treatment against cancer and prevention of virus infections. Heparan sulfate, an analog of heparin, which is not used for medical purposes yet, was reported to have the same pharmaceutical potential as heparin. Both heparin and heparan sulfate share a common precursor molecule known as heparosan. Heparosan determines the polymer chain length and the sugar unit backbone composition, which are determinant structural parameters for the biological activity of heparin and heparan sulfate. In this review we give an overview of the different methods used to synthesize heparosan, and we highlight the pro and cons of each method in respect to the synthesis of bioengineered heparin-like molecules. Advancements in the field of the synthesis of bioengineered heparin are also reported.

KW - n-deacetylase/n-sulfotransferase

KW - k5 capsular polysaccharide

KW - multocida type-a

KW - escherichia-coli

KW - pasteurella-multocida

KW - chemoenzymatic synthesis

KW - molecular-weight

KW - hyaluronic-acid

KW - sulfate/heparin biosynthesis

KW - bioengineered heparin

U2 - 10.1016/j.carbpol.2012.04.046

DO - 10.1016/j.carbpol.2012.04.046

M3 - Article

VL - 93

SP - 38

EP - 47

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

IS - 1

ER -