Probe mobility in native phosphocaseinate suspensions and in a concentrated rennet gel: effect of probe flexibility and size

S. Salami, C. Rondeau-Mouro, J.P.M. van Duynhoven, F. Mariette

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

Pulsed field gradient nuclear magnetic resonance and proton nuclear magnetic resonance relaxometry were used to study the self-diffusion coefficients and molecular dynamics of linear (PEGs) and spherical probes (dendrimers) in native phosphocaseinate suspensions and in a concentrated rennet gel. It was shown that both the size and the shape of the diffusing molecules and the matrix topography affected the diffusion and relaxation rates. In suspensions, both translational and rotational diffusion decreased with increasing casein concentrations due to increased restriction in the freedom of motion. Rotational diffusion was, however, less hindered than translational diffusion. After coagulation, translational diffusion increased but rotational diffusion decreased. Analysis of the T2 relaxation times obtained for probes of different sizes distinguished the free short-chain relaxation formed from a few monomeric units from (i) the relaxation of protons attached to long polymer chains and (ii) the short-chain relaxation attached to a rigid dendrimer core.
Original languageEnglish
Pages (from-to)5870-5879
JournalJournal of Agricultural and Food Chemistry
Volume61
Issue number24
DOIs
Publication statusPublished - 2013

Fingerprint

rennet
Suspensions
Gels
gels
Dendrimers
Nuclear magnetic resonance
protons
nuclear magnetic resonance spectroscopy
Protons
Magnetic Resonance Spectroscopy
molecular dynamics
coagulation
diffusivity
Molecular Dynamics Simulation
topography
Caseins
Coagulation
casein
polymers
Relaxation time

Keywords

  • diffusing wave spectroscopy
  • glucono-delta-lactone
  • nmr self-diffusion
  • casein micelles
  • skim milk
  • rheological properties
  • pfg-nmr
  • electron-microscopy
  • poly(vinyl alcohol)
  • induced aggregation

Cite this

@article{655624f9606d49e48ab62bce14c29104,
title = "Probe mobility in native phosphocaseinate suspensions and in a concentrated rennet gel: effect of probe flexibility and size",
abstract = "Pulsed field gradient nuclear magnetic resonance and proton nuclear magnetic resonance relaxometry were used to study the self-diffusion coefficients and molecular dynamics of linear (PEGs) and spherical probes (dendrimers) in native phosphocaseinate suspensions and in a concentrated rennet gel. It was shown that both the size and the shape of the diffusing molecules and the matrix topography affected the diffusion and relaxation rates. In suspensions, both translational and rotational diffusion decreased with increasing casein concentrations due to increased restriction in the freedom of motion. Rotational diffusion was, however, less hindered than translational diffusion. After coagulation, translational diffusion increased but rotational diffusion decreased. Analysis of the T2 relaxation times obtained for probes of different sizes distinguished the free short-chain relaxation formed from a few monomeric units from (i) the relaxation of protons attached to long polymer chains and (ii) the short-chain relaxation attached to a rigid dendrimer core.",
keywords = "diffusing wave spectroscopy, glucono-delta-lactone, nmr self-diffusion, casein micelles, skim milk, rheological properties, pfg-nmr, electron-microscopy, poly(vinyl alcohol), induced aggregation",
author = "S. Salami and C. Rondeau-Mouro and {van Duynhoven}, J.P.M. and F. Mariette",
year = "2013",
doi = "10.1021/jf304949c",
language = "English",
volume = "61",
pages = "5870--5879",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "24",

}

Probe mobility in native phosphocaseinate suspensions and in a concentrated rennet gel: effect of probe flexibility and size. / Salami, S.; Rondeau-Mouro, C.; van Duynhoven, J.P.M.; Mariette, F.

In: Journal of Agricultural and Food Chemistry, Vol. 61, No. 24, 2013, p. 5870-5879.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Probe mobility in native phosphocaseinate suspensions and in a concentrated rennet gel: effect of probe flexibility and size

AU - Salami, S.

AU - Rondeau-Mouro, C.

AU - van Duynhoven, J.P.M.

AU - Mariette, F.

PY - 2013

Y1 - 2013

N2 - Pulsed field gradient nuclear magnetic resonance and proton nuclear magnetic resonance relaxometry were used to study the self-diffusion coefficients and molecular dynamics of linear (PEGs) and spherical probes (dendrimers) in native phosphocaseinate suspensions and in a concentrated rennet gel. It was shown that both the size and the shape of the diffusing molecules and the matrix topography affected the diffusion and relaxation rates. In suspensions, both translational and rotational diffusion decreased with increasing casein concentrations due to increased restriction in the freedom of motion. Rotational diffusion was, however, less hindered than translational diffusion. After coagulation, translational diffusion increased but rotational diffusion decreased. Analysis of the T2 relaxation times obtained for probes of different sizes distinguished the free short-chain relaxation formed from a few monomeric units from (i) the relaxation of protons attached to long polymer chains and (ii) the short-chain relaxation attached to a rigid dendrimer core.

AB - Pulsed field gradient nuclear magnetic resonance and proton nuclear magnetic resonance relaxometry were used to study the self-diffusion coefficients and molecular dynamics of linear (PEGs) and spherical probes (dendrimers) in native phosphocaseinate suspensions and in a concentrated rennet gel. It was shown that both the size and the shape of the diffusing molecules and the matrix topography affected the diffusion and relaxation rates. In suspensions, both translational and rotational diffusion decreased with increasing casein concentrations due to increased restriction in the freedom of motion. Rotational diffusion was, however, less hindered than translational diffusion. After coagulation, translational diffusion increased but rotational diffusion decreased. Analysis of the T2 relaxation times obtained for probes of different sizes distinguished the free short-chain relaxation formed from a few monomeric units from (i) the relaxation of protons attached to long polymer chains and (ii) the short-chain relaxation attached to a rigid dendrimer core.

KW - diffusing wave spectroscopy

KW - glucono-delta-lactone

KW - nmr self-diffusion

KW - casein micelles

KW - skim milk

KW - rheological properties

KW - pfg-nmr

KW - electron-microscopy

KW - poly(vinyl alcohol)

KW - induced aggregation

U2 - 10.1021/jf304949c

DO - 10.1021/jf304949c

M3 - Article

VL - 61

SP - 5870

EP - 5879

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 24

ER -