Primary Separation: 2-D Electrophoresis

R.P. Pedreschi Plasencia

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    The advancements in two-dimensional electrophoresis (2DE) have consolidated it as a key tool for gel-based proteomics applications. Nowadays, 2DE is extensively applied and it is a useful technique for the simultaneous separation of hundreds to thousands of proteins, analysis, and differential quantification especially for the study of nonmodel organisms. This chapter focuses on all the steps involved in standard 2DE in protein expression. The pros and cons of the technique are discussed as well as potential variants for special applications. Special attention is given to data pre-processing and statistical analysis of 2DE generated data in order to draw sound conclusions from biological studies.
    Original languageEnglish
    Title of host publicationProteomics in Foods: Principles and Applications
    EditorsF. Toldrá, L.M.L. Nollet
    Place of PublicationNew York, USA
    Pages51-59
    Number of pages589
    DOIs
    Publication statusPublished - 2013

    Publication series

    NameFood Microbiology and Food Safety
    PublisherSpringer
    Number2

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