Prevalence of tick-borne viruses in Ixodes ricinus assessed by high-throughput real-time PCR

Mathilde Gondard, Lorraine Michelet, Athinna Nisavanh, Elodie Devillers, Sabine Delannoy, Patrick Fach, Anna Aspan, Karin Ullman, Jan Chirico, Bernd Hoffmann, Fimme Jan van der Wal, Aline de Koeijer, Conny van Solt-Smits, Seta Jahfari, Hein Sprong, Karen L. Mansfield, Anthony R. Fooks, Kirstine Klitgaard, Rene Bødker, Sara Moutailler

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Ticks are one of the principal arthropod vectors of human and animal infectious diseases. Whereas the prevalence of tick-borne encephalitis virus in ticks in Europe is well studied, there is less information available on the prevalence of the other tick-borne viruses (TBVs) existing worldwide. The aim of this study was to improve the epidemiological survey tools of TBVs by the development of an efficient high-throughput test to screen a wide range of viruses in ticks. In this study, we developed a new high-throughput virus-detection assay based on parallel real-time PCRs on a microfluidic system, and used it to perform a large scale epidemiological survey screening for the presence of 21 TBVs in 18 135 nymphs of Ixodes ricinus collected from five European countries. This extensive investigation has (i) evaluated the prevalence of four viruses present in the collected ticks, (ii) allowed the identification of viruses in regions where they were previously undetected. In conclusion, we have demonstrated the capabilities of this new screening method that allows the detection of numerous TBVs in a large number of ticks. This tool represents a powerful and rapid system for TBVs surveillance in Europe and could be easily customized to assess viral emergence.

Original languageEnglish
Article numberfty083
Journal Pathogens and Disease
Volume76
Issue number8
DOIs
Publication statusPublished - Nov 2018

Fingerprint

Ixodes
Ticks
Real-Time Polymerase Chain Reaction
Viruses
Arthropod Vectors
Tick-Borne Encephalitis Viruses
Nymph
Animal Diseases
Microfluidics
Communicable Diseases

Keywords

  • Europe
  • microfluidic analysis
  • molecular epidemiology
  • surveillance
  • tick borne viruses

Cite this

Gondard, M., Michelet, L., Nisavanh, A., Devillers, E., Delannoy, S., Fach, P., ... Moutailler, S. (2018). Prevalence of tick-borne viruses in Ixodes ricinus assessed by high-throughput real-time PCR. Pathogens and Disease, 76(8), [fty083]. https://doi.org/10.1093/femspd/fty083
Gondard, Mathilde ; Michelet, Lorraine ; Nisavanh, Athinna ; Devillers, Elodie ; Delannoy, Sabine ; Fach, Patrick ; Aspan, Anna ; Ullman, Karin ; Chirico, Jan ; Hoffmann, Bernd ; van der Wal, Fimme Jan ; de Koeijer, Aline ; van Solt-Smits, Conny ; Jahfari, Seta ; Sprong, Hein ; Mansfield, Karen L. ; Fooks, Anthony R. ; Klitgaard, Kirstine ; Bødker, Rene ; Moutailler, Sara. / Prevalence of tick-borne viruses in Ixodes ricinus assessed by high-throughput real-time PCR. In: Pathogens and Disease. 2018 ; Vol. 76, No. 8.
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abstract = "Ticks are one of the principal arthropod vectors of human and animal infectious diseases. Whereas the prevalence of tick-borne encephalitis virus in ticks in Europe is well studied, there is less information available on the prevalence of the other tick-borne viruses (TBVs) existing worldwide. The aim of this study was to improve the epidemiological survey tools of TBVs by the development of an efficient high-throughput test to screen a wide range of viruses in ticks. In this study, we developed a new high-throughput virus-detection assay based on parallel real-time PCRs on a microfluidic system, and used it to perform a large scale epidemiological survey screening for the presence of 21 TBVs in 18 135 nymphs of Ixodes ricinus collected from five European countries. This extensive investigation has (i) evaluated the prevalence of four viruses present in the collected ticks, (ii) allowed the identification of viruses in regions where they were previously undetected. In conclusion, we have demonstrated the capabilities of this new screening method that allows the detection of numerous TBVs in a large number of ticks. This tool represents a powerful and rapid system for TBVs surveillance in Europe and could be easily customized to assess viral emergence.",
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Gondard, M, Michelet, L, Nisavanh, A, Devillers, E, Delannoy, S, Fach, P, Aspan, A, Ullman, K, Chirico, J, Hoffmann, B, van der Wal, FJ, de Koeijer, A, van Solt-Smits, C, Jahfari, S, Sprong, H, Mansfield, KL, Fooks, AR, Klitgaard, K, Bødker, R & Moutailler, S 2018, 'Prevalence of tick-borne viruses in Ixodes ricinus assessed by high-throughput real-time PCR' Pathogens and Disease, vol. 76, no. 8, fty083. https://doi.org/10.1093/femspd/fty083

Prevalence of tick-borne viruses in Ixodes ricinus assessed by high-throughput real-time PCR. / Gondard, Mathilde; Michelet, Lorraine; Nisavanh, Athinna; Devillers, Elodie; Delannoy, Sabine; Fach, Patrick; Aspan, Anna; Ullman, Karin; Chirico, Jan; Hoffmann, Bernd; van der Wal, Fimme Jan; de Koeijer, Aline; van Solt-Smits, Conny; Jahfari, Seta; Sprong, Hein; Mansfield, Karen L.; Fooks, Anthony R.; Klitgaard, Kirstine; Bødker, Rene; Moutailler, Sara.

In: Pathogens and Disease, Vol. 76, No. 8, fty083, 11.2018.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Prevalence of tick-borne viruses in Ixodes ricinus assessed by high-throughput real-time PCR

AU - Gondard, Mathilde

AU - Michelet, Lorraine

AU - Nisavanh, Athinna

AU - Devillers, Elodie

AU - Delannoy, Sabine

AU - Fach, Patrick

AU - Aspan, Anna

AU - Ullman, Karin

AU - Chirico, Jan

AU - Hoffmann, Bernd

AU - van der Wal, Fimme Jan

AU - de Koeijer, Aline

AU - van Solt-Smits, Conny

AU - Jahfari, Seta

AU - Sprong, Hein

AU - Mansfield, Karen L.

AU - Fooks, Anthony R.

AU - Klitgaard, Kirstine

AU - Bødker, Rene

AU - Moutailler, Sara

PY - 2018/11

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N2 - Ticks are one of the principal arthropod vectors of human and animal infectious diseases. Whereas the prevalence of tick-borne encephalitis virus in ticks in Europe is well studied, there is less information available on the prevalence of the other tick-borne viruses (TBVs) existing worldwide. The aim of this study was to improve the epidemiological survey tools of TBVs by the development of an efficient high-throughput test to screen a wide range of viruses in ticks. In this study, we developed a new high-throughput virus-detection assay based on parallel real-time PCRs on a microfluidic system, and used it to perform a large scale epidemiological survey screening for the presence of 21 TBVs in 18 135 nymphs of Ixodes ricinus collected from five European countries. This extensive investigation has (i) evaluated the prevalence of four viruses present in the collected ticks, (ii) allowed the identification of viruses in regions where they were previously undetected. In conclusion, we have demonstrated the capabilities of this new screening method that allows the detection of numerous TBVs in a large number of ticks. This tool represents a powerful and rapid system for TBVs surveillance in Europe and could be easily customized to assess viral emergence.

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KW - Europe

KW - microfluidic analysis

KW - molecular epidemiology

KW - surveillance

KW - tick borne viruses

U2 - 10.1093/femspd/fty083

DO - 10.1093/femspd/fty083

M3 - Article

VL - 76

JO - Pathogens and Disease

JF - Pathogens and Disease

SN - 2049-632X

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ER -

Gondard M, Michelet L, Nisavanh A, Devillers E, Delannoy S, Fach P et al. Prevalence of tick-borne viruses in Ixodes ricinus assessed by high-throughput real-time PCR. Pathogens and Disease. 2018 Nov;76(8). fty083. https://doi.org/10.1093/femspd/fty083