Prevalence of phylogroups and O25/ST131 insusceptible and extended-spectrum β-lactamase-producing Escherichia coli isolates, the Netherlands

I.T.M.A. Overdevest; A.M.C. Bergmans; J.J. Verweij; J. Vissers; N. Bax; E. Snelders; J.A.J.W. Kluytmans

doi:10.1016/j.cmi.2015.02.020

Prevalence of phylogroups and O25/ST131 insusceptible and extended-spectrum β-lactamase-producing Escherichia coli isolates, the Netherlands

I.T.M.A. Overdevest^*, A.M.C. Bergmans, J.J. Verweij, J. Vissers, N. Bax, E. Snelders, J.A.J.W. Kluytmans

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

8 Citations (Scopus)

Abstract

To assess the distribution of phylogroups and O25/ST131 in the Netherlands, we performed a real-time polymerase chain reaction (PCR) on a collection of 108 wild-type Escherichia coli (WT-EC) and 134 extended-spectrum β-lactamase-producing E. coli (ESBL-EC). Phylogroup B2 was predominant, but ESBL-EC were less likely to belong to this phylogroup (48.5%) than were WT-EC (66.7%; p=0.005). In WT-EC, phylogroups B2 and D seem to be more virulent, having a higher prevalence among midstream urine isolates and blood culture isolates, than in catheter-related urine isolates (83.3% and 87.9% vs. 61.9%; p 0.048). O25/ST131 is associated with ESBL production, being almost absent among phylogroup B2 WT-EC (61.5% vs. 5.6%; p<0.001).

Original language	English
Pages (from-to)	570.e1-570.e4
Journal	Clinical Microbiology and Infection
Volume	21
Issue number	6
DOIs	https://doi.org/10.1016/j.cmi.2015.02.020
Publication status	Published - 1 Jun 2015
Externally published	Yes

Keywords

Blood culture
E.coli
Phylogroups
Rectal colonization
ST131
Urine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Overdevest, I. T. M. A., Bergmans, A. M. C., Verweij, J. J., Vissers, J., Bax, N., Snelders, E., & Kluytmans, J. A. J. W. (2015). Prevalence of phylogroups and O25/ST131 insusceptible and extended-spectrum β-lactamase-producing Escherichia coli isolates, the Netherlands. Clinical Microbiology and Infection, 21(6), 570.e1-570.e4. https://doi.org/10.1016/j.cmi.2015.02.020

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title = "Prevalence of phylogroups and O25/ST131 insusceptible and extended-spectrum β-lactamase-producing Escherichia coli isolates, the Netherlands",

abstract = "To assess the distribution of phylogroups and O25/ST131 in the Netherlands, we performed a real-time polymerase chain reaction (PCR) on a collection of 108 wild-type Escherichia coli (WT-EC) and 134 extended-spectrum β-lactamase-producing E. coli (ESBL-EC). Phylogroup B2 was predominant, but ESBL-EC were less likely to belong to this phylogroup (48.5\%) than were WT-EC (66.7\%; p=0.005). In WT-EC, phylogroups B2 and D seem to be more virulent, having a higher prevalence among midstream urine isolates and blood culture isolates, than in catheter-related urine isolates (83.3\% and 87.9\% vs. 61.9\%; p 0.048). O25/ST131 is associated with ESBL production, being almost absent among phylogroup B2 WT-EC (61.5\% vs. 5.6\%; p<0.001).",

keywords = "Blood culture, E.coli, Phylogroups, Rectal colonization, ST131, Urine",

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Prevalence of phylogroups and O25/ST131 insusceptible and extended-spectrum β-lactamase-producing Escherichia coli isolates, the Netherlands. / Overdevest, I.T.M.A.; Bergmans, A.M.C.; Verweij, J.J. et al.
In: Clinical Microbiology and Infection, Vol. 21, No. 6, 01.06.2015, p. 570.e1-570.e4.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Prevalence of phylogroups and O25/ST131 insusceptible and extended-spectrum β-lactamase-producing Escherichia coli isolates, the Netherlands

AU - Overdevest, I.T.M.A.

AU - Bergmans, A.M.C.

AU - Verweij, J.J.

AU - Vissers, J.

AU - Bax, N.

AU - Snelders, E.

AU - Kluytmans, J.A.J.W.

PY - 2015/6/1

Y1 - 2015/6/1

N2 - To assess the distribution of phylogroups and O25/ST131 in the Netherlands, we performed a real-time polymerase chain reaction (PCR) on a collection of 108 wild-type Escherichia coli (WT-EC) and 134 extended-spectrum β-lactamase-producing E. coli (ESBL-EC). Phylogroup B2 was predominant, but ESBL-EC were less likely to belong to this phylogroup (48.5%) than were WT-EC (66.7%; p=0.005). In WT-EC, phylogroups B2 and D seem to be more virulent, having a higher prevalence among midstream urine isolates and blood culture isolates, than in catheter-related urine isolates (83.3% and 87.9% vs. 61.9%; p 0.048). O25/ST131 is associated with ESBL production, being almost absent among phylogroup B2 WT-EC (61.5% vs. 5.6%; p<0.001).

AB - To assess the distribution of phylogroups and O25/ST131 in the Netherlands, we performed a real-time polymerase chain reaction (PCR) on a collection of 108 wild-type Escherichia coli (WT-EC) and 134 extended-spectrum β-lactamase-producing E. coli (ESBL-EC). Phylogroup B2 was predominant, but ESBL-EC were less likely to belong to this phylogroup (48.5%) than were WT-EC (66.7%; p=0.005). In WT-EC, phylogroups B2 and D seem to be more virulent, having a higher prevalence among midstream urine isolates and blood culture isolates, than in catheter-related urine isolates (83.3% and 87.9% vs. 61.9%; p 0.048). O25/ST131 is associated with ESBL production, being almost absent among phylogroup B2 WT-EC (61.5% vs. 5.6%; p<0.001).

KW - Blood culture

KW - E.coli

KW - Phylogroups

KW - Rectal colonization

KW - ST131

KW - Urine

U2 - 10.1016/j.cmi.2015.02.020

DO - 10.1016/j.cmi.2015.02.020

M3 - Article

C2 - 25749563

AN - SCOPUS:84930822706

SN - 1198-743X

VL - 21

SP - 570.e1-570.e4

JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

IS - 6

ER -

Prevalence of phylogroups and O25/ST131 insusceptible and extended-spectrum β-lactamase-producing Escherichia coli isolates, the Netherlands

Abstract

Keywords

UN SDGs

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