Prevalence of Leptospira spp. and Seoul hantavirus in brown rats (Rattus norvegicus) in four regions in the Netherlands, 2011-2015

Miriam Maas, Ankje De Vries, Chantal Reusken, Jan Buijs, Marga Goris, Rudy Hartskeerl, Ahmed Ahmed, Peter van Tulden, Arno Swart, Roan Pijnacker, Miriam Koene, Åke Lundkvist, Paul Heyman, Barry Rockx, Joke Van Der Giessen

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4 Citations (Scopus)

Abstract

Background:  Brown rats (Rattus norvegicus) may carry pathogens that can be a risk for public health. Brown rats in the Netherlands were tested for the zoonotic pathogens Leptospira spp. and Seoul hantavirus (SEOV), in order to obtain insight in their prevalence. Methods and results:  Cross-sectional studies were performed at four locations from 2011 to 2015. The rats were tested for Leptospira spp. using real-time PCR and/or culture resulting in a prevalence ranging between 33–57%. Testing for SEOV was done through an adapted human Seoul hantavirus ELISA and real-time RT-PCR. Although at several locations the ELISA indicated presence of SEOV antibodies, none could be confirmed by focus reduction neutralization testing. Conclusion:  The results indicate a widespread presence of Leptospira spp. in brown rats in the Netherlands, including areas with a low leptospirosis incidence in humans. No evidence for circulation of SEOV was found in this study.

Original languageEnglish
Article number1490135
JournalInfection Ecology and Epidemiology
Volume8
Issue number1
DOIs
Publication statusPublished - 26 Jun 2018

Fingerprint

Hantavirus
Leptospira
Netherlands
pathogen
neutralization
antibody
public health
Real-Time Polymerase Chain Reaction
Enzyme-Linked Immunosorbent Assay
Leptospirosis
Zoonoses
Public Health
Cross-Sectional Studies
Seoul
Antibodies
Incidence
leptospirosis
method

Keywords

  • epidemiology
  • hantavirus
  • Leptospirosis
  • prevalence
  • Rattus norvegicus
  • Seoul virus

Cite this

Maas, Miriam ; De Vries, Ankje ; Reusken, Chantal ; Buijs, Jan ; Goris, Marga ; Hartskeerl, Rudy ; Ahmed, Ahmed ; van Tulden, Peter ; Swart, Arno ; Pijnacker, Roan ; Koene, Miriam ; Lundkvist, Åke ; Heyman, Paul ; Rockx, Barry ; Van Der Giessen, Joke. / Prevalence of Leptospira spp. and Seoul hantavirus in brown rats (Rattus norvegicus) in four regions in the Netherlands, 2011-2015. In: Infection Ecology and Epidemiology. 2018 ; Vol. 8, No. 1.
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title = "Prevalence of Leptospira spp. and Seoul hantavirus in brown rats (Rattus norvegicus) in four regions in the Netherlands, 2011-2015",
abstract = "Background:  Brown rats (Rattus norvegicus) may carry pathogens that can be a risk for public health. Brown rats in the Netherlands were tested for the zoonotic pathogens Leptospira spp. and Seoul hantavirus (SEOV), in order to obtain insight in their prevalence. Methods and results:  Cross-sectional studies were performed at four locations from 2011 to 2015. The rats were tested for Leptospira spp. using real-time PCR and/or culture resulting in a prevalence ranging between 33–57{\%}. Testing for SEOV was done through an adapted human Seoul hantavirus ELISA and real-time RT-PCR. Although at several locations the ELISA indicated presence of SEOV antibodies, none could be confirmed by focus reduction neutralization testing. Conclusion:  The results indicate a widespread presence of Leptospira spp. in brown rats in the Netherlands, including areas with a low leptospirosis incidence in humans. No evidence for circulation of SEOV was found in this study.",
keywords = "epidemiology, hantavirus, Leptospirosis, prevalence, Rattus norvegicus, Seoul virus",
author = "Miriam Maas and {De Vries}, Ankje and Chantal Reusken and Jan Buijs and Marga Goris and Rudy Hartskeerl and Ahmed Ahmed and {van Tulden}, Peter and Arno Swart and Roan Pijnacker and Miriam Koene and {\AA}ke Lundkvist and Paul Heyman and Barry Rockx and {Van Der Giessen}, Joke",
year = "2018",
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day = "26",
doi = "10.1080/20008686.2018.1490135",
language = "English",
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Maas, M, De Vries, A, Reusken, C, Buijs, J, Goris, M, Hartskeerl, R, Ahmed, A, van Tulden, P, Swart, A, Pijnacker, R, Koene, M, Lundkvist, Å, Heyman, P, Rockx, B & Van Der Giessen, J 2018, 'Prevalence of Leptospira spp. and Seoul hantavirus in brown rats (Rattus norvegicus) in four regions in the Netherlands, 2011-2015' Infection Ecology and Epidemiology, vol. 8, no. 1, 1490135. https://doi.org/10.1080/20008686.2018.1490135

Prevalence of Leptospira spp. and Seoul hantavirus in brown rats (Rattus norvegicus) in four regions in the Netherlands, 2011-2015. / Maas, Miriam; De Vries, Ankje; Reusken, Chantal; Buijs, Jan; Goris, Marga; Hartskeerl, Rudy; Ahmed, Ahmed; van Tulden, Peter; Swart, Arno; Pijnacker, Roan; Koene, Miriam; Lundkvist, Åke; Heyman, Paul; Rockx, Barry; Van Der Giessen, Joke.

In: Infection Ecology and Epidemiology, Vol. 8, No. 1, 1490135, 26.06.2018.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Prevalence of Leptospira spp. and Seoul hantavirus in brown rats (Rattus norvegicus) in four regions in the Netherlands, 2011-2015

AU - Maas, Miriam

AU - De Vries, Ankje

AU - Reusken, Chantal

AU - Buijs, Jan

AU - Goris, Marga

AU - Hartskeerl, Rudy

AU - Ahmed, Ahmed

AU - van Tulden, Peter

AU - Swart, Arno

AU - Pijnacker, Roan

AU - Koene, Miriam

AU - Lundkvist, Åke

AU - Heyman, Paul

AU - Rockx, Barry

AU - Van Der Giessen, Joke

PY - 2018/6/26

Y1 - 2018/6/26

N2 - Background:  Brown rats (Rattus norvegicus) may carry pathogens that can be a risk for public health. Brown rats in the Netherlands were tested for the zoonotic pathogens Leptospira spp. and Seoul hantavirus (SEOV), in order to obtain insight in their prevalence. Methods and results:  Cross-sectional studies were performed at four locations from 2011 to 2015. The rats were tested for Leptospira spp. using real-time PCR and/or culture resulting in a prevalence ranging between 33–57%. Testing for SEOV was done through an adapted human Seoul hantavirus ELISA and real-time RT-PCR. Although at several locations the ELISA indicated presence of SEOV antibodies, none could be confirmed by focus reduction neutralization testing. Conclusion:  The results indicate a widespread presence of Leptospira spp. in brown rats in the Netherlands, including areas with a low leptospirosis incidence in humans. No evidence for circulation of SEOV was found in this study.

AB - Background:  Brown rats (Rattus norvegicus) may carry pathogens that can be a risk for public health. Brown rats in the Netherlands were tested for the zoonotic pathogens Leptospira spp. and Seoul hantavirus (SEOV), in order to obtain insight in their prevalence. Methods and results:  Cross-sectional studies were performed at four locations from 2011 to 2015. The rats were tested for Leptospira spp. using real-time PCR and/or culture resulting in a prevalence ranging between 33–57%. Testing for SEOV was done through an adapted human Seoul hantavirus ELISA and real-time RT-PCR. Although at several locations the ELISA indicated presence of SEOV antibodies, none could be confirmed by focus reduction neutralization testing. Conclusion:  The results indicate a widespread presence of Leptospira spp. in brown rats in the Netherlands, including areas with a low leptospirosis incidence in humans. No evidence for circulation of SEOV was found in this study.

KW - epidemiology

KW - hantavirus

KW - Leptospirosis

KW - prevalence

KW - Rattus norvegicus

KW - Seoul virus

U2 - 10.1080/20008686.2018.1490135

DO - 10.1080/20008686.2018.1490135

M3 - Article

VL - 8

JO - Infection Ecology and Epidemiology

JF - Infection Ecology and Epidemiology

SN - 2000-8686

IS - 1

M1 - 1490135

ER -