TY - JOUR
T1 - Preterm prelabor rupture of membranes (PPROM) is not associated with presence of viral genomes in the amniotic fluid
AU - Bopegamage, Shubhada
AU - Kacerovsky, Marian
AU - Tambor, Vojtech
AU - Musilova, Ivana
AU - Sarmirova, Sona
AU - Snelders, Eveline
AU - de Jong, Arjan S.
AU - Vari, Sandor G.
AU - Melchers, Willem J.G.
AU - Galama, Jochem M.D.
PY - 2013/11
Y1 - 2013/11
N2 - Background: The role of viral infections in preterm prelabor rupture of the membranes (PPROM) is not established. Studies on the presence of viral genomes in the amniotic fluid (AF) collected in pregnancies complicated by PPROM show contradictory outcomes. Objectives: To investigate AF samples of PPROM pregnancies for the presence of viral genomes. Study design: AF samples from patients with PPROM were collected during a 4-year (2008-2012) observational study. 174 women were included with selection criteria of singleton pregnancy, PPROM, and maternal age of 18 years and above. PCR was used for detection of human cytomegalovirus (HCMV), herpes simplex virus (HSV), parvovirus B19, human adenoviruses (HAdV), enteroviruses (EV) and human parechovirus (HPeV). The selection of these viral targets was based on literature regarding screening of AF for presence of viral genomes. Results: Only a single sample was positive out of the 174 tested AFs, HCMV DNA was detected. Conclusions: PPROM is not associated with active viral infections.
AB - Background: The role of viral infections in preterm prelabor rupture of the membranes (PPROM) is not established. Studies on the presence of viral genomes in the amniotic fluid (AF) collected in pregnancies complicated by PPROM show contradictory outcomes. Objectives: To investigate AF samples of PPROM pregnancies for the presence of viral genomes. Study design: AF samples from patients with PPROM were collected during a 4-year (2008-2012) observational study. 174 women were included with selection criteria of singleton pregnancy, PPROM, and maternal age of 18 years and above. PCR was used for detection of human cytomegalovirus (HCMV), herpes simplex virus (HSV), parvovirus B19, human adenoviruses (HAdV), enteroviruses (EV) and human parechovirus (HPeV). The selection of these viral targets was based on literature regarding screening of AF for presence of viral genomes. Results: Only a single sample was positive out of the 174 tested AFs, HCMV DNA was detected. Conclusions: PPROM is not associated with active viral infections.
KW - Amniotic fluid
KW - Pregnancy
KW - Pregnancy outcome
KW - Premature rupture of membranes
KW - Viral genomes
U2 - 10.1016/j.jcv.2013.09.013
DO - 10.1016/j.jcv.2013.09.013
M3 - Article
C2 - 24113293
AN - SCOPUS:84886296167
SN - 1386-6532
VL - 58
SP - 559
EP - 563
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
IS - 3
ER -
