Pressurized Liquid Extraction of Pigments from Chlamydomonas sp. and Chemical Characterization by HPLC–MS/MS

Lidia Montero; Mahshid Sedghi; Yolanda García; Carlos Almeida; Carl Safi; Nicole Engelen-Smit; Alejandro Cifuentes; Jose A. Mendiola; Elena Ibáñez

doi:10.1007/s41664-018-0062-8

Pressurized Liquid Extraction of Pigments from Chlamydomonas sp. and Chemical Characterization by HPLC–MS/MS

Lidia Montero, Mahshid Sedghi, Yolanda García, Carlos Almeida, Carl Safi, Nicole Engelen-Smit, Alejandro Cifuentes, Jose A. Mendiola^*, Elena Ibáñez

^*Corresponding author for this work

Biorefinery & Fibre Techn

Research output: Contribution to journal › Article › Academic › peer-review

13 Citations (Scopus)

Abstract

Pressurized liquid extraction using green solvents (ethanol and water) was applied to extract photosynthetic pigments from the microalgae Chlamydomonas sp. An experimental design is employed to determine the effect of the extraction conditions on the extraction yield and the antioxidant capacity of the different extracts determined using 1,1-diphenyl-2-picryl-hydrazyl and TEAC tests. All the extracts show significant antioxidant activity in all assays. Besides, in this work, the carotenoid and chlorophyll composition of the Chlamydomonas extracts was analyzed by HPLC–DAD–APCI–MS/MS for the first time, being the main compounds lutein and pheophythin a´.

Original language	English
Pages (from-to)	149-157
Number of pages	9
Journal	Journal of Analysis and Testing
Volume	2
Issue number	2
DOIs	https://doi.org/10.1007/s41664-018-0062-8
Publication status	Published - 17 Aug 2018

Keywords

Antioxidant capacity
Carotenoids
Chlamydomonas sp
HPLC–DAD–APCI–MS/MS
Pigments
Pressurized liquid extraction

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10.1007/s41664-018-0062-8

MIRACLES: Multi-product Integrated bioRefinery of Algae: from Carbon dioxide and Light Energy to high-value Specialties
1/11/13 → 31/10/17
Project: EU research project

Cite this

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title = "Pressurized Liquid Extraction of Pigments from Chlamydomonas sp. and Chemical Characterization by HPLC–MS/MS",

abstract = "Pressurized liquid extraction using green solvents (ethanol and water) was applied to extract photosynthetic pigments from the microalgae Chlamydomonas sp. An experimental design is employed to determine the effect of the extraction conditions on the extraction yield and the antioxidant capacity of the different extracts determined using 1,1-diphenyl-2-picryl-hydrazyl and TEAC tests. All the extracts show significant antioxidant activity in all assays. Besides, in this work, the carotenoid and chlorophyll composition of the Chlamydomonas extracts was analyzed by HPLC–DAD–APCI–MS/MS for the first time, being the main compounds lutein and pheophythin a´.",

keywords = "Antioxidant capacity, Carotenoids, Chlamydomonas sp, HPLC–DAD–APCI–MS/MS, Pigments, Pressurized liquid extraction",

author = "Lidia Montero and Mahshid Sedghi and Yolanda Garc{\'i}a and Carlos Almeida and Carl Safi and Nicole Engelen-Smit and Alejandro Cifuentes and Mendiola, {Jose A.} and Elena Ib{\'a}{\~n}ez",

year = "2018",

month = aug,

day = "17",

doi = "10.1007/s41664-018-0062-8",

language = "English",

volume = "2",

pages = "149--157",

journal = "Journal of Analysis and Testing",

issn = "2509-4696",

publisher = "Springer",

number = "2",

}

TY - JOUR

T1 - Pressurized Liquid Extraction of Pigments from Chlamydomonas sp. and Chemical Characterization by HPLC–MS/MS

AU - Montero, Lidia

AU - Sedghi, Mahshid

AU - García, Yolanda

AU - Almeida, Carlos

AU - Safi, Carl

AU - Engelen-Smit, Nicole

AU - Cifuentes, Alejandro

AU - Mendiola, Jose A.

AU - Ibáñez, Elena

PY - 2018/8/17

Y1 - 2018/8/17

N2 - Pressurized liquid extraction using green solvents (ethanol and water) was applied to extract photosynthetic pigments from the microalgae Chlamydomonas sp. An experimental design is employed to determine the effect of the extraction conditions on the extraction yield and the antioxidant capacity of the different extracts determined using 1,1-diphenyl-2-picryl-hydrazyl and TEAC tests. All the extracts show significant antioxidant activity in all assays. Besides, in this work, the carotenoid and chlorophyll composition of the Chlamydomonas extracts was analyzed by HPLC–DAD–APCI–MS/MS for the first time, being the main compounds lutein and pheophythin a´.

AB - Pressurized liquid extraction using green solvents (ethanol and water) was applied to extract photosynthetic pigments from the microalgae Chlamydomonas sp. An experimental design is employed to determine the effect of the extraction conditions on the extraction yield and the antioxidant capacity of the different extracts determined using 1,1-diphenyl-2-picryl-hydrazyl and TEAC tests. All the extracts show significant antioxidant activity in all assays. Besides, in this work, the carotenoid and chlorophyll composition of the Chlamydomonas extracts was analyzed by HPLC–DAD–APCI–MS/MS for the first time, being the main compounds lutein and pheophythin a´.

KW - Antioxidant capacity

KW - Carotenoids

KW - Chlamydomonas sp

KW - HPLC–DAD–APCI–MS/MS

KW - Pigments

KW - Pressurized liquid extraction

U2 - 10.1007/s41664-018-0062-8

DO - 10.1007/s41664-018-0062-8

M3 - Article

AN - SCOPUS:85069715339

SN - 2509-4696

VL - 2

SP - 149

EP - 157

JO - Journal of Analysis and Testing

JF - Journal of Analysis and Testing

IS - 2

ER -

Pressurized Liquid Extraction of Pigments from Chlamydomonas sp. and Chemical Characterization by HPLC–MS/MS

Abstract

Keywords

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Projects

MIRACLES: Multi-product Integrated bioRefinery of Algae: from Carbon dioxide and Light Energy to high-value Specialties

Cite this