Preservation of Meloidogyne hapla and M. chitwoodi in liquid nitrogen: Differences in response between populations

J.G. van der Beek; W.B.J. Veldhuis; C. ZijIstra; C.H. van Silfhout

Preservation of Meloidogyne hapla and M. chitwoodi in liquid nitrogen: Differences in response between populations

J.G. van der Beek, W.B.J. Veldhuis, C. ZijIstra, C.H. van Silfhout

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A procedure for long-term preservation of gennplasm of Meloidogyne hapla and M. chitwoodi in liquid nitrogen is described, including a pretrearrnenr with 10% ethanediol for 2 h at room temperature and 40 % ethanecliol for 45 min on ice. Survival rates ranged from 45 to 98 % with an average of 75 %. Comparison of three different populations of M. hapla, two populations of M. chitwoodi and two populations of Meloidogyne n. sp. revealed a significantly higher survival for one M. hapla population, whiJe the survival rates of the other six were not significantly different. Ir was shown that higher lipid reserves in juveniles could possibly explain the high survival of this M. hapla population. Juveniles after freezing were able to reproduce on plants, but infectivity was significantly lower than of non-frozen juveniles. Ir is recommended to multiply juveniles, stored in liquid nitrogen, for one generation before being used as inoculum for experimentation

Original language	English
Pages (from-to)	227-234
Journal	Fundamental and applied nematology
Volume	19
Issue number	3
Publication status	Published - 1996

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@article{a18e687bf36d4529be89047cdb4c8b61,

title = "Preservation of Meloidogyne hapla and M. chitwoodi in liquid nitrogen: Differences in response between populations",

abstract = "A procedure for long-term preservation of gennplasm of Meloidogyne hapla and M. chitwoodi in liquid nitrogen is described, including a pretrearrnenr with 10% ethanediol for 2 h at room temperature and 40 % ethanecliol for 45 min on ice. Survival rates ranged from 45 to 98 % with an average of 75 %. Comparison of three different populations of M. hapla, two populations of M. chitwoodi and two populations of Meloidogyne n. sp. revealed a significantly higher survival for one M. hapla population, whiJe the survival rates of the other six were not significantly different. Ir was shown that higher lipid reserves in juveniles could possibly explain the high survival of this M. hapla population. Juveniles after freezing were able to reproduce on plants, but infectivity was significantly lower than of non-frozen juveniles. Ir is recommended to multiply juveniles, stored in liquid nitrogen, for one generation before being used as inoculum for experimentation",

author = "{van der Beek}, J.G. and W.B.J. Veldhuis and C. ZijIstra and {van Silfhout}, C.H.",

year = "1996",

language = "English",

volume = "19",

pages = "227--234",

journal = "Fundamental and applied nematology",

issn = "1164-5571",

publisher = "Brill",

number = "3",

}

TY - JOUR

T1 - Preservation of Meloidogyne hapla and M. chitwoodi in liquid nitrogen: Differences in response between populations

AU - van der Beek, J.G.

AU - Veldhuis, W.B.J.

AU - ZijIstra, C.

AU - van Silfhout, C.H.

PY - 1996

Y1 - 1996

N2 - A procedure for long-term preservation of gennplasm of Meloidogyne hapla and M. chitwoodi in liquid nitrogen is described, including a pretrearrnenr with 10% ethanediol for 2 h at room temperature and 40 % ethanecliol for 45 min on ice. Survival rates ranged from 45 to 98 % with an average of 75 %. Comparison of three different populations of M. hapla, two populations of M. chitwoodi and two populations of Meloidogyne n. sp. revealed a significantly higher survival for one M. hapla population, whiJe the survival rates of the other six were not significantly different. Ir was shown that higher lipid reserves in juveniles could possibly explain the high survival of this M. hapla population. Juveniles after freezing were able to reproduce on plants, but infectivity was significantly lower than of non-frozen juveniles. Ir is recommended to multiply juveniles, stored in liquid nitrogen, for one generation before being used as inoculum for experimentation

AB - A procedure for long-term preservation of gennplasm of Meloidogyne hapla and M. chitwoodi in liquid nitrogen is described, including a pretrearrnenr with 10% ethanediol for 2 h at room temperature and 40 % ethanecliol for 45 min on ice. Survival rates ranged from 45 to 98 % with an average of 75 %. Comparison of three different populations of M. hapla, two populations of M. chitwoodi and two populations of Meloidogyne n. sp. revealed a significantly higher survival for one M. hapla population, whiJe the survival rates of the other six were not significantly different. Ir was shown that higher lipid reserves in juveniles could possibly explain the high survival of this M. hapla population. Juveniles after freezing were able to reproduce on plants, but infectivity was significantly lower than of non-frozen juveniles. Ir is recommended to multiply juveniles, stored in liquid nitrogen, for one generation before being used as inoculum for experimentation

M3 - Article

VL - 19

SP - 227

EP - 234

JO - Fundamental and applied nematology

JF - Fundamental and applied nematology

SN - 1164-5571

IS - 3

ER -