Preservation of Meloidogyne hapla and M. chitwoodi in liquid nitrogen: Differences in response between populations

J.G. van der Beek, W.B.J. Veldhuis, C. ZijIstra, C.H. van Silfhout

    Research output: Contribution to journalArticleAcademicpeer-review

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    Abstract

    A procedure for long-term preservation of gennplasm of Meloidogyne hapla and M. chitwoodi in liquid nitrogen is described, including a pretrearrnenr with 10% ethanediol for 2 h at room temperature and 40 % ethanecliol for 45 min on ice. Survival rates ranged from 45 to 98 % with an average of 75 %. Comparison of three different populations of M. hapla, two populations of M. chitwoodi and two populations of Meloidogyne n. sp. revealed a significantly higher survival for one M. hapla population, whiJe the survival rates of the other six were not significantly different. Ir was shown that higher lipid reserves in juveniles could possibly explain the high survival of this M. hapla population. Juveniles after freezing were able to reproduce on plants, but infectivity was significantly lower than of non-frozen juveniles. Ir is recommended to multiply juveniles, stored in liquid nitrogen, for one generation before being used as inoculum for experimentation
    Original languageEnglish
    Pages (from-to)227-234
    JournalFundamental and applied nematology
    Volume19
    Issue number3
    Publication statusPublished - 1996

    Fingerprint

    Meloidogyne chitwoodi
    Tylenchoidea
    Meloidogyne hapla
    Nitrogen
    liquid
    liquids
    nitrogen
    Population
    Ethylene Glycols
    survival rate
    infectivity
    Meloidogyne
    freezing
    Ice
    lipid
    Freezing
    ambient temperature
    inoculum
    ice
    pathogenicity

    Cite this

    van der Beek, J. G., Veldhuis, W. B. J., ZijIstra, C., & van Silfhout, C. H. (1996). Preservation of Meloidogyne hapla and M. chitwoodi in liquid nitrogen: Differences in response between populations. Fundamental and applied nematology, 19(3), 227-234.
    van der Beek, J.G. ; Veldhuis, W.B.J. ; ZijIstra, C. ; van Silfhout, C.H. / Preservation of Meloidogyne hapla and M. chitwoodi in liquid nitrogen: Differences in response between populations. In: Fundamental and applied nematology. 1996 ; Vol. 19, No. 3. pp. 227-234.
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    title = "Preservation of Meloidogyne hapla and M. chitwoodi in liquid nitrogen: Differences in response between populations",
    abstract = "A procedure for long-term preservation of gennplasm of Meloidogyne hapla and M. chitwoodi in liquid nitrogen is described, including a pretrearrnenr with 10{\%} ethanediol for 2 h at room temperature and 40 {\%} ethanecliol for 45 min on ice. Survival rates ranged from 45 to 98 {\%} with an average of 75 {\%}. Comparison of three different populations of M. hapla, two populations of M. chitwoodi and two populations of Meloidogyne n. sp. revealed a significantly higher survival for one M. hapla population, whiJe the survival rates of the other six were not significantly different. Ir was shown that higher lipid reserves in juveniles could possibly explain the high survival of this M. hapla population. Juveniles after freezing were able to reproduce on plants, but infectivity was significantly lower than of non-frozen juveniles. Ir is recommended to multiply juveniles, stored in liquid nitrogen, for one generation before being used as inoculum for experimentation",
    author = "{van der Beek}, J.G. and W.B.J. Veldhuis and C. ZijIstra and {van Silfhout}, C.H.",
    year = "1996",
    language = "English",
    volume = "19",
    pages = "227--234",
    journal = "Fundamental and applied nematology",
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    van der Beek, JG, Veldhuis, WBJ, ZijIstra, C & van Silfhout, CH 1996, 'Preservation of Meloidogyne hapla and M. chitwoodi in liquid nitrogen: Differences in response between populations', Fundamental and applied nematology, vol. 19, no. 3, pp. 227-234.

    Preservation of Meloidogyne hapla and M. chitwoodi in liquid nitrogen: Differences in response between populations. / van der Beek, J.G.; Veldhuis, W.B.J.; ZijIstra, C.; van Silfhout, C.H.

    In: Fundamental and applied nematology, Vol. 19, No. 3, 1996, p. 227-234.

    Research output: Contribution to journalArticleAcademicpeer-review

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    AU - van der Beek, J.G.

    AU - Veldhuis, W.B.J.

    AU - ZijIstra, C.

    AU - van Silfhout, C.H.

    PY - 1996

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    N2 - A procedure for long-term preservation of gennplasm of Meloidogyne hapla and M. chitwoodi in liquid nitrogen is described, including a pretrearrnenr with 10% ethanediol for 2 h at room temperature and 40 % ethanecliol for 45 min on ice. Survival rates ranged from 45 to 98 % with an average of 75 %. Comparison of three different populations of M. hapla, two populations of M. chitwoodi and two populations of Meloidogyne n. sp. revealed a significantly higher survival for one M. hapla population, whiJe the survival rates of the other six were not significantly different. Ir was shown that higher lipid reserves in juveniles could possibly explain the high survival of this M. hapla population. Juveniles after freezing were able to reproduce on plants, but infectivity was significantly lower than of non-frozen juveniles. Ir is recommended to multiply juveniles, stored in liquid nitrogen, for one generation before being used as inoculum for experimentation

    AB - A procedure for long-term preservation of gennplasm of Meloidogyne hapla and M. chitwoodi in liquid nitrogen is described, including a pretrearrnenr with 10% ethanediol for 2 h at room temperature and 40 % ethanecliol for 45 min on ice. Survival rates ranged from 45 to 98 % with an average of 75 %. Comparison of three different populations of M. hapla, two populations of M. chitwoodi and two populations of Meloidogyne n. sp. revealed a significantly higher survival for one M. hapla population, whiJe the survival rates of the other six were not significantly different. Ir was shown that higher lipid reserves in juveniles could possibly explain the high survival of this M. hapla population. Juveniles after freezing were able to reproduce on plants, but infectivity was significantly lower than of non-frozen juveniles. Ir is recommended to multiply juveniles, stored in liquid nitrogen, for one generation before being used as inoculum for experimentation

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