Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 227-234 |
| Journal | Fundamental and applied nematology |
| Volume | 19 |
| Issue number | 3 |
| Publication status | Published - 1996 |
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Preservation of Meloidogyne hapla and M. chitwoodi in liquid nitrogen: Differences in response between populations. / van der Beek, J.G.; Veldhuis, W.B.J.; ZijIstra, C.; van Silfhout, C.H.
In: Fundamental and applied nematology, Vol. 19, No. 3, 1996, p. 227-234.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Preservation of Meloidogyne hapla and M. chitwoodi in liquid nitrogen: Differences in response between populations
AU - van der Beek, J.G.
AU - Veldhuis, W.B.J.
AU - ZijIstra, C.
AU - van Silfhout, C.H.
PY - 1996
Y1 - 1996
N2 - A procedure for long-term preservation of gennplasm of Meloidogyne hapla and M. chitwoodi in liquid nitrogen is described, including a pretrearrnenr with 10% ethanediol for 2 h at room temperature and 40 % ethanecliol for 45 min on ice. Survival rates ranged from 45 to 98 % with an average of 75 %. Comparison of three different populations of M. hapla, two populations of M. chitwoodi and two populations of Meloidogyne n. sp. revealed a significantly higher survival for one M. hapla population, whiJe the survival rates of the other six were not significantly different. Ir was shown that higher lipid reserves in juveniles could possibly explain the high survival of this M. hapla population. Juveniles after freezing were able to reproduce on plants, but infectivity was significantly lower than of non-frozen juveniles. Ir is recommended to multiply juveniles, stored in liquid nitrogen, for one generation before being used as inoculum for experimentation
AB - A procedure for long-term preservation of gennplasm of Meloidogyne hapla and M. chitwoodi in liquid nitrogen is described, including a pretrearrnenr with 10% ethanediol for 2 h at room temperature and 40 % ethanecliol for 45 min on ice. Survival rates ranged from 45 to 98 % with an average of 75 %. Comparison of three different populations of M. hapla, two populations of M. chitwoodi and two populations of Meloidogyne n. sp. revealed a significantly higher survival for one M. hapla population, whiJe the survival rates of the other six were not significantly different. Ir was shown that higher lipid reserves in juveniles could possibly explain the high survival of this M. hapla population. Juveniles after freezing were able to reproduce on plants, but infectivity was significantly lower than of non-frozen juveniles. Ir is recommended to multiply juveniles, stored in liquid nitrogen, for one generation before being used as inoculum for experimentation
M3 - Article
VL - 19
SP - 227
EP - 234
JO - Fundamental and applied nematology
JF - Fundamental and applied nematology
SN - 1164-5571
IS - 3
ER -