Preservation of Meloidogyne hapla and M. chitwoodi in liquid nitrogen: Differences in response between populations

J.G. van der Beek, W.B.J. Veldhuis, C. ZijIstra, C.H. van Silfhout

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    Abstract

    A procedure for long-term preservation of gennplasm of Meloidogyne hapla and M. chitwoodi in liquid nitrogen is described, including a pretrearrnenr with 10% ethanediol for 2 h at room temperature and 40 % ethanecliol for 45 min on ice. Survival rates ranged from 45 to 98 % with an average of 75 %. Comparison of three different populations of M. hapla, two populations of M. chitwoodi and two populations of Meloidogyne n. sp. revealed a significantly higher survival for one M. hapla population, whiJe the survival rates of the other six were not significantly different. Ir was shown that higher lipid reserves in juveniles could possibly explain the high survival of this M. hapla population. Juveniles after freezing were able to reproduce on plants, but infectivity was significantly lower than of non-frozen juveniles. Ir is recommended to multiply juveniles, stored in liquid nitrogen, for one generation before being used as inoculum for experimentation
    Original languageEnglish
    Pages (from-to)227-234
    JournalFundamental and applied nematology
    Volume19
    Issue number3
    Publication statusPublished - 1996

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