Presence of an intron in inverted repeat constructs does not necessarily have an effect on efficiency of post-transcriptional gene silencing

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

The effect of introns on silencing efficiency was tested in inverted repeat constructs of Granule-Bound Starch Synthase (GBSSI) cDNA by comparing the silencing efficiencies induced by inverted repeat constructs with and without introns. No effect could be attributed to the presence of introns indicating that the introns neither enhance nor inhibit post-transcriptional gene silencing. The effect of a spliceable intron in the spacer was studied by comparing constructs harbouring a spliceable or a non-spliceable intron in the spacer. As opposed to the general belief that splicing of an intron increases silencing efficiency, the use of a spliceable intron in the spacer did not result in enhancement of silencing in our experimental system
Original languageEnglish
Pages (from-to)307-316
JournalMolecular Breeding
Volume17
Issue number4
DOIs
Publication statusPublished - 2006

Keywords

  • bound starch synthase
  • double-stranded-rna
  • solanum-tuberosum-l
  • mediated enhancement
  • antisense rna
  • maize cells
  • expression
  • potato
  • granule
  • interference

Fingerprint Dive into the research topics of 'Presence of an intron in inverted repeat constructs does not necessarily have an effect on efficiency of post-transcriptional gene silencing'. Together they form a unique fingerprint.

Cite this