Prenatal developmental toxicity testing of petroleum substances

Application of the mouse embryonic stem cell test (EST) to compare in vitro potencies with potencies observed in vivo

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

Prenatal developmental toxicity (PDT) as observed with some petroleum substances (PS) has been associated with the presence of 3–7 ring polycyclic aromatic hydrocarbons (PAHs). In the present study, the applicability of ES-D3 cell differentiation assay of the EST to evaluate in vitro embryotoxicity potencies of PS and gas-to-liquid (GTL) products as compared to their in vivo potencies was investigated. DMSO-extracts of a range of PS, containing different amounts of PAHs, and GTL-products, which are devoid of PAHs, were tested in the ES-D3 cell proliferation and differentiation assays of the EST. The results show that PS inhibited the differentiation of ES-D3 cells into cardiomyocytes in a concentration-dependent manner at non-cytotoxic concentrations, and that their potency was proportional to their PAH content. In contrast, as expected, GTL-products did not inhibit ES-D3 cell viability or differentiation at all. The in vitro PDT potencies were compared to published in vivo PDT studies, and a good correlation was found between in vitro and in vivo results (R2 = 0.97). To conclude, our results support the hypothesis that PAHs are the primary inducers of the PDT in PS.
Original languageEnglish
Pages (from-to)303-312
JournalToxicology in Vitro
Volume44
DOIs
Publication statusPublished - 2017

Fingerprint

Polycyclic Aromatic Hydrocarbons
Petroleum
Stem cells
Toxicity
Testing
Cell Differentiation
Gases
Embryonic Stem Cells
Assays
Liquids
Cell proliferation
Dimethyl Sulfoxide
Cardiac Myocytes
Cell Survival
Cells
Cell Proliferation
In Vitro Techniques
Mouse Embryonic Stem Cells

Keywords

  • Embryonic stem cell test
  • Gas-to-liquid products
  • Petroleum substances
  • Polycyclic aromatic hydrocarbons
  • Prenatal developmental toxicity
  • UVCBs

Cite this

@article{6afbff2cac6a464ca5d0e71a38167752,
title = "Prenatal developmental toxicity testing of petroleum substances: Application of the mouse embryonic stem cell test (EST) to compare in vitro potencies with potencies observed in vivo",
abstract = "Prenatal developmental toxicity (PDT) as observed with some petroleum substances (PS) has been associated with the presence of 3–7 ring polycyclic aromatic hydrocarbons (PAHs). In the present study, the applicability of ES-D3 cell differentiation assay of the EST to evaluate in vitro embryotoxicity potencies of PS and gas-to-liquid (GTL) products as compared to their in vivo potencies was investigated. DMSO-extracts of a range of PS, containing different amounts of PAHs, and GTL-products, which are devoid of PAHs, were tested in the ES-D3 cell proliferation and differentiation assays of the EST. The results show that PS inhibited the differentiation of ES-D3 cells into cardiomyocytes in a concentration-dependent manner at non-cytotoxic concentrations, and that their potency was proportional to their PAH content. In contrast, as expected, GTL-products did not inhibit ES-D3 cell viability or differentiation at all. The in vitro PDT potencies were compared to published in vivo PDT studies, and a good correlation was found between in vitro and in vivo results (R2 = 0.97). To conclude, our results support the hypothesis that PAHs are the primary inducers of the PDT in PS.",
keywords = "Embryonic stem cell test, Gas-to-liquid products, Petroleum substances, Polycyclic aromatic hydrocarbons, Prenatal developmental toxicity, UVCBs",
author = "Lenny Kamelia and Jochem Louisse and {de Haan}, Laura and Rietjens, {Ivonne M.C.M.} and Boogaard, {Peter J.}",
year = "2017",
doi = "10.1016/j.tiv.2017.07.018",
language = "English",
volume = "44",
pages = "303--312",
journal = "Toxicology in Vitro",
issn = "0887-2333",
publisher = "Elsevier",

}

TY - JOUR

T1 - Prenatal developmental toxicity testing of petroleum substances

T2 - Application of the mouse embryonic stem cell test (EST) to compare in vitro potencies with potencies observed in vivo

AU - Kamelia, Lenny

AU - Louisse, Jochem

AU - de Haan, Laura

AU - Rietjens, Ivonne M.C.M.

AU - Boogaard, Peter J.

PY - 2017

Y1 - 2017

N2 - Prenatal developmental toxicity (PDT) as observed with some petroleum substances (PS) has been associated with the presence of 3–7 ring polycyclic aromatic hydrocarbons (PAHs). In the present study, the applicability of ES-D3 cell differentiation assay of the EST to evaluate in vitro embryotoxicity potencies of PS and gas-to-liquid (GTL) products as compared to their in vivo potencies was investigated. DMSO-extracts of a range of PS, containing different amounts of PAHs, and GTL-products, which are devoid of PAHs, were tested in the ES-D3 cell proliferation and differentiation assays of the EST. The results show that PS inhibited the differentiation of ES-D3 cells into cardiomyocytes in a concentration-dependent manner at non-cytotoxic concentrations, and that their potency was proportional to their PAH content. In contrast, as expected, GTL-products did not inhibit ES-D3 cell viability or differentiation at all. The in vitro PDT potencies were compared to published in vivo PDT studies, and a good correlation was found between in vitro and in vivo results (R2 = 0.97). To conclude, our results support the hypothesis that PAHs are the primary inducers of the PDT in PS.

AB - Prenatal developmental toxicity (PDT) as observed with some petroleum substances (PS) has been associated with the presence of 3–7 ring polycyclic aromatic hydrocarbons (PAHs). In the present study, the applicability of ES-D3 cell differentiation assay of the EST to evaluate in vitro embryotoxicity potencies of PS and gas-to-liquid (GTL) products as compared to their in vivo potencies was investigated. DMSO-extracts of a range of PS, containing different amounts of PAHs, and GTL-products, which are devoid of PAHs, were tested in the ES-D3 cell proliferation and differentiation assays of the EST. The results show that PS inhibited the differentiation of ES-D3 cells into cardiomyocytes in a concentration-dependent manner at non-cytotoxic concentrations, and that their potency was proportional to their PAH content. In contrast, as expected, GTL-products did not inhibit ES-D3 cell viability or differentiation at all. The in vitro PDT potencies were compared to published in vivo PDT studies, and a good correlation was found between in vitro and in vivo results (R2 = 0.97). To conclude, our results support the hypothesis that PAHs are the primary inducers of the PDT in PS.

KW - Embryonic stem cell test

KW - Gas-to-liquid products

KW - Petroleum substances

KW - Polycyclic aromatic hydrocarbons

KW - Prenatal developmental toxicity

KW - UVCBs

U2 - 10.1016/j.tiv.2017.07.018

DO - 10.1016/j.tiv.2017.07.018

M3 - Article

VL - 44

SP - 303

EP - 312

JO - Toxicology in Vitro

JF - Toxicology in Vitro

SN - 0887-2333

ER -