Preliminary evaluation of a lateral flow immunoassay device for screening urine samples for the presence of sulphamethazine

M. O'Keeffe; P. Crabbe; M. Salden; J. Wichers; C. van Peteghem; F. Kohen; G. Pieraccini

doi:10.1016/S0022-1759(03)00207-2

Preliminary evaluation of a lateral flow immunoassay device for screening urine samples for the presence of sulphamethazine

M. O'Keeffe, P. Crabbe, M. Salden, J. Wichers, C. van Peteghem, F. Kohen, G. Pieraccini

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

A lateral flow immunoassay (LFIA) device was developed and applied to testing urine samples for residues of the antimicrobial sulphamethazine (SMZ). This report describes the preparation of a rat monoclonal antibody to SMZ and its characterisation in an ELISA format. Apart from SMZ, the antibody showed high (50¿cross-reactivity to N4-acetyl-sulphamethazine (55¿ sulphamerazine (59¿and sulphisoxazole (50¿and lower cross-reactivity of 18 o sulphachlorpyridazine and sulphadiazine. The LFIA device consisted of a nitrocellulose membrane spotted with SMZ-ovalbumin and goat anti-mouse antibody as capture line and control line, respectively. Mouse anti-rat IgG F(ab')2 fragment specific antibody, adsorbed to colloidal carbon, was used as the detection ligand in the LFIA. The LFIA device had a cut-off value of 6.3 ng/ml in diluted (1/10) urine. Urine samples from SMZ-treated pigs, and bovine and porcine urine samples fortified with SMZ were used for a blind, four-laboratory evaluation of the performance of the LFIA device. Concentrations of SMZ in the test samples (n=29), as determined by LC-MS/MS, ranged from 0 (

Original language	English
Pages (from-to)	117-126
Journal	Journal of Immunological Methods
Volume	278
Issue number	1-2
DOIs	https://doi.org/10.1016/S0022-1759(03)00207-2
Publication status	Published - 2003

Keywords

enzyme-immunoassay
swine urine
predictors
tissues

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10.1016/S0022-1759(03)00207-2

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title = "Preliminary evaluation of a lateral flow immunoassay device for screening urine samples for the presence of sulphamethazine",

abstract = "A lateral flow immunoassay (LFIA) device was developed and applied to testing urine samples for residues of the antimicrobial sulphamethazine (SMZ). This report describes the preparation of a rat monoclonal antibody to SMZ and its characterisation in an ELISA format. Apart from SMZ, the antibody showed high (50¿cross-reactivity to N4-acetyl-sulphamethazine (55¿ sulphamerazine (59¿and sulphisoxazole (50¿and lower cross-reactivity of 18 o sulphachlorpyridazine and sulphadiazine. The LFIA device consisted of a nitrocellulose membrane spotted with SMZ-ovalbumin and goat anti-mouse antibody as capture line and control line, respectively. Mouse anti-rat IgG F(ab')2 fragment specific antibody, adsorbed to colloidal carbon, was used as the detection ligand in the LFIA. The LFIA device had a cut-off value of 6.3 ng/ml in diluted (1/10) urine. Urine samples from SMZ-treated pigs, and bovine and porcine urine samples fortified with SMZ were used for a blind, four-laboratory evaluation of the performance of the LFIA device. Concentrations of SMZ in the test samples (n=29), as determined by LC-MS/MS, ranged from 0 (",

keywords = "enzyme-immunoassay, swine urine, predictors, tissues",

author = "M. O'Keeffe and P. Crabbe and M. Salden and J. Wichers and {van Peteghem}, C. and F. Kohen and G. Pieraccini",

year = "2003",

doi = "10.1016/S0022-1759(03)00207-2",

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AU - O'Keeffe, M.

AU - Crabbe, P.

AU - Salden, M.

AU - Wichers, J.

AU - van Peteghem, C.

AU - Kohen, F.

AU - Pieraccini, G.

PY - 2003

Y1 - 2003

N2 - A lateral flow immunoassay (LFIA) device was developed and applied to testing urine samples for residues of the antimicrobial sulphamethazine (SMZ). This report describes the preparation of a rat monoclonal antibody to SMZ and its characterisation in an ELISA format. Apart from SMZ, the antibody showed high (50¿cross-reactivity to N4-acetyl-sulphamethazine (55¿ sulphamerazine (59¿and sulphisoxazole (50¿and lower cross-reactivity of 18 o sulphachlorpyridazine and sulphadiazine. The LFIA device consisted of a nitrocellulose membrane spotted with SMZ-ovalbumin and goat anti-mouse antibody as capture line and control line, respectively. Mouse anti-rat IgG F(ab')2 fragment specific antibody, adsorbed to colloidal carbon, was used as the detection ligand in the LFIA. The LFIA device had a cut-off value of 6.3 ng/ml in diluted (1/10) urine. Urine samples from SMZ-treated pigs, and bovine and porcine urine samples fortified with SMZ were used for a blind, four-laboratory evaluation of the performance of the LFIA device. Concentrations of SMZ in the test samples (n=29), as determined by LC-MS/MS, ranged from 0 (

AB - A lateral flow immunoassay (LFIA) device was developed and applied to testing urine samples for residues of the antimicrobial sulphamethazine (SMZ). This report describes the preparation of a rat monoclonal antibody to SMZ and its characterisation in an ELISA format. Apart from SMZ, the antibody showed high (50¿cross-reactivity to N4-acetyl-sulphamethazine (55¿ sulphamerazine (59¿and sulphisoxazole (50¿and lower cross-reactivity of 18 o sulphachlorpyridazine and sulphadiazine. The LFIA device consisted of a nitrocellulose membrane spotted with SMZ-ovalbumin and goat anti-mouse antibody as capture line and control line, respectively. Mouse anti-rat IgG F(ab')2 fragment specific antibody, adsorbed to colloidal carbon, was used as the detection ligand in the LFIA. The LFIA device had a cut-off value of 6.3 ng/ml in diluted (1/10) urine. Urine samples from SMZ-treated pigs, and bovine and porcine urine samples fortified with SMZ were used for a blind, four-laboratory evaluation of the performance of the LFIA device. Concentrations of SMZ in the test samples (n=29), as determined by LC-MS/MS, ranged from 0 (

KW - enzyme-immunoassay

KW - swine urine

KW - predictors

KW - tissues

U2 - 10.1016/S0022-1759(03)00207-2

DO - 10.1016/S0022-1759(03)00207-2

M3 - Article

SN - 0022-1759

VL - 278

SP - 117

EP - 126

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1-2

ER -

Preliminary evaluation of a lateral flow immunoassay device for screening urine samples for the presence of sulphamethazine

Abstract

Keywords

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