Preliminary evaluation of a lateral flow immunoassay device for screening urine samples for the presence of sulphamethazine

M. O'Keeffe, P. Crabbe, M. Salden, J. Wichers, C. van Peteghem, F. Kohen, G. Pieraccini

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    Abstract

    A lateral flow immunoassay (LFIA) device was developed and applied to testing urine samples for residues of the antimicrobial sulphamethazine (SMZ). This report describes the preparation of a rat monoclonal antibody to SMZ and its characterisation in an ELISA format. Apart from SMZ, the antibody showed high (50¿cross-reactivity to N4-acetyl-sulphamethazine (55¿ sulphamerazine (59¿and sulphisoxazole (50¿and lower cross-reactivity of 18 o sulphachlorpyridazine and sulphadiazine. The LFIA device consisted of a nitrocellulose membrane spotted with SMZ-ovalbumin and goat anti-mouse antibody as capture line and control line, respectively. Mouse anti-rat IgG F(ab')2 fragment specific antibody, adsorbed to colloidal carbon, was used as the detection ligand in the LFIA. The LFIA device had a cut-off value of 6.3 ng/ml in diluted (1/10) urine. Urine samples from SMZ-treated pigs, and bovine and porcine urine samples fortified with SMZ were used for a blind, four-laboratory evaluation of the performance of the LFIA device. Concentrations of SMZ in the test samples (n=29), as determined by LC-MS/MS, ranged from 0 (
    Original languageEnglish
    Pages (from-to)117-126
    JournalJournal of Immunological Methods
    Volume278
    Issue number1-2
    DOIs
    Publication statusPublished - 2003

    Keywords

    • enzyme-immunoassay
    • swine urine
    • predictors
    • tissues

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