Prebiotic utilisation provides Lactiplantibacillus plantarum a competitive advantage in vitro, but is not reflected by an increased intestinal fitness

Jori Fuhren, Markus Schwalbe, Jos Boekhorst, Christiane Rösch, Henk A. Schols, Michiel Kleerebezem*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Synbiotics combine the concepts of probiotics and prebiotics to synergistically enhance the health-associated effects of both components. Previously, we have shown that the intestinal persistence of inulin-utilizing L. plantarum Lp900 is significantly increased in rats fed an inulin-supplemented, high-calcium diet. Here we employed a competitive population dynamics approach to demonstrate that inulin and GOS can selectively enrich L. plantarum strains that utilize these substrates for growth during in vitro cultivation, but that such enrichment did not occur during intestinal transit in rats fed a GOS or inulin-supplemented diet. The intestinal persistence of all L. plantarum strains increased irrespective of their prebiotic utilization phenotype, which was dependent on the calcium level of the diet. Analysis of fecal microbiota and intestinal persistence decline rates indicated that prebiotic utilization capacity did not selectively stimulate intestinal persistence in prebiotic supplemented diets. Moreover, microbiota and organic acid profile analyses indicate that the prebiotic utilizing probiotic strains are vastly outcompeted by the endogenous prebiotic-utilizing microbiota, and that the collective enhanced persistence of all L. plantarum strains is most likely explained by their well-established tolerance to organic acids.

Original languageEnglish
Article number2338946
JournalGut Microbes
Volume16
Issue number1
DOIs
Publication statusPublished - 24 Apr 2024

Keywords

  • competition
  • galacto-oligosaccharides
  • intestinal persistence
  • inulin
  • Lactiplantibacillus plantarum
  • prebiotics
  • probiotics

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