Practical use of corrected fluorescence excitation and emission spectra of fluorescent proteins in Förster Resonance Energy Transfer (FRET) studies

M.A. Hink, N.V. Visser, J.W. Borst, A. van Hoek, A.J.W.G. Visser

Research output: Contribution to journalArticleAcademicpeer-review

47 Citations (Scopus)

Abstract

Corrected fluorescence excitation and emission spectra have been obtained from several enhanced variants of the green fluorescent protein (EGFP) isolated from the jellyfish Aequorea victoria, blue fluorescence protein (EBFP), cyan fluorescent protein (ECFP), EGFP and yellow fluorescent protein (EYFP-citrine) and from the red fluorescent protein (DsRed) isolated from the coral species Discosoma. The spectra are stored in a database. This report describes how the spectra can be used as templates to derive the critical transfer distance for any pair of fluorescent proteins.
Original languageEnglish
Pages (from-to)185-188
JournalJournal of Fluorescence
Volume13
DOIs
Publication statusPublished - 2003

Keywords

  • living cells
  • microscopy
  • biochemistry

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