PPP1, a plant-specific regulator of transcription controls Arabidopsis development and PIN expression

René Benjamins*, Elke Barbez, Martina Ortbauer, Inez Terpstra, Doris Lucyshyn, Jeanette Moulinier-Anzola, Muhammad Asaf Khan, Johannes Leitner, Nenad Malenica, Haroon Butt, Barbara Korbei, Ben Scheres, Jürgen Kleine-Vehn, Christian Luschnig

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Directional transport of auxin is essential for plant development, with PIN auxin transport proteins representing an integral part of the machinery that controls hormone distribution. However, unlike the rapidly emerging framework of molecular determinants regulating PIN protein abundance and subcellular localization, insights into mechanisms controlling PIN transcription are still limited. Here we describe PIN2 PROMOTER BINDING PROTEIN 1 (PPP1), an evolutionary conserved plant-specific DNA binding protein that acts on transcription of PIN genes. Consistent with PPP1 DNA-binding activity, PPP1 reporter proteins are nuclear localized and analysis of PPP1 null alleles and knockdown lines indicated a function as a positive regulator of PIN expression. Furthermore, we show that ppp1 pleiotropic mutant phenotypes are partially reverted by PIN overexpression, and results are presented that underline a role of PPP1-PIN promoter interaction in PIN expression control. Collectively, our findings identify an elementary, thus far unknown, plant-specific DNA-binding protein required for post-embryonic plant development, in general, and correct expression of PIN genes, in particular.

Original languageEnglish
Article number32196
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016

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    Benjamins, R., Barbez, E., Ortbauer, M., Terpstra, I., Lucyshyn, D., Moulinier-Anzola, J., ... Luschnig, C. (2016). PPP1, a plant-specific regulator of transcription controls Arabidopsis development and PIN expression. Scientific Reports, 6, [32196]. https://doi.org/10.1038/srep32196