Powdery Mildew Resistance in Tomato by Impairment of SIPMR4 and SIDMR1

R.P. Huibers, A.E.H.M. Loonen, Dongli Dongli Gao, G. van den Ackerveken, R.G.F. Visser, Y. Bai

Research output: Contribution to journalArticleAcademicpeer-review

29 Citations (Scopus)

Abstract

Genetic dissection of disease susceptibility in Arabidopsis to powdery and downy mildew has identified multiple susceptibility (S) genes whose impairment results in disease resistance. Although several of these S-genes have been cloned and characterized in more detail it is unknown to which degree their function in disease susceptibility is conserved among different plant species. Moreover, it is unclear whether impairment of such genes has potential in disease resistance breeding due to possible fitness costs associated with impaired alleles. Here we show that the Arabidopsis PMR4 and DMR1, genes encoding a callose synthase and homoserine kinase respectively, have functional orthologs in tomato with respect to their S-gene function. Silencing of both genes using RNAi resulted in resistance to the tomato powdery mildew fungus Oidium neolycopersici. Resistance to O. neolycopersici by SlDMR1 silencing was associated with severely reduced plant growth whereas SlPMR4 silencing was not. SlPMR4 is therefore a suitable candidate gene as target for mutagenesis to obtain alleles that can be deployed in disease resistance breeding of tomato.
Original languageEnglish
Article numbere67467
JournalPLoS ONE
Volume8
Issue number6
DOIs
Publication statusPublished - 2013

Fingerprint

Lycopersicon esculentum
powdery mildew
Genes
disease resistance
tomatoes
Disease Resistance
Oidium neolycopersici
genes
Disease Susceptibility
homoserine kinase
Arabidopsis
Breeding
Alleles
1,3-beta-glucan synthase
alleles
Dissection
Mutagenesis
Inborn Genetic Diseases
Gene encoding
downy mildew

Keywords

  • zinc-finger nucleases
  • disease resistance
  • arabidopsis mutants
  • targeted mutagenesis
  • gene
  • plants
  • pathogenesis
  • mutation

Cite this

Huibers, R.P. ; Loonen, A.E.H.M. ; Dongli Gao, Dongli ; van den Ackerveken, G. ; Visser, R.G.F. ; Bai, Y. / Powdery Mildew Resistance in Tomato by Impairment of SIPMR4 and SIDMR1. In: PLoS ONE. 2013 ; Vol. 8, No. 6.
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Powdery Mildew Resistance in Tomato by Impairment of SIPMR4 and SIDMR1. / Huibers, R.P.; Loonen, A.E.H.M.; Dongli Gao, Dongli; van den Ackerveken, G.; Visser, R.G.F.; Bai, Y.

In: PLoS ONE, Vol. 8, No. 6, e67467, 2013.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Huibers, R.P.

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AU - van den Ackerveken, G.

AU - Visser, R.G.F.

AU - Bai, Y.

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