Potential of Treatment-Specific Protein Biomarker Profiles for Detection of Hormone Abuse in Cattle

S.K.J. Ludwig, N.G.E. Smits, F.T. Cannizzo, M.W.F. Nielen

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

Targeted protein biomarker profiling is suggested as a fast screening approach for detection of illegal hormone treatment in meat production. The advantage of using biomarkers is that they mark the biological response and, thus, are responsive to a panel of substances with similar effects. In a preliminary feasibility study, a 4-plex protein biomarker flow cytometric immunoassay (FCIA) previously developed for the detection of recombinant bovine somatotropin (rbST) was applied to cattle treated with steroids, such as estradiol, dexamethasone, and prednisolone. Each treatment resulted in a specific plasma biomarker profile for insulin-like growth factor-1 (IGF-1), IGF binding protein 2, osteocalcin, and anti-rbST antibodies, which could be distinguished from the profile of untreated animals. In summary, the 4-plex biomarker FCIA is, apart from rbST, also capable of detecting treatment with other growth-promoting agents and therefore clearly shows the potential of biomarker profiling as a screening method in veterinary control. It is proposed to perform additional validation studies covering high numbers of treated and untreated animals to support inclusion or adaptation of protein biomarker approaches in future monitoring regulations.
Original languageEnglish
Pages (from-to)4514-4519
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume61
Issue number19
DOIs
Publication statusPublished - 2013

Fingerprint

Biomarkers
biomarkers
hormones
Hormones
cattle
Proteins
somatotropin
Growth Hormone
proteins
immunoassays
Immunoassay
Screening
Animals
Insulin-Like Growth Factor Binding Protein 2
screening
osteocalcin
prednisolone
insulin-like growth factor binding proteins
Meats
Validation Studies

Keywords

  • recombinant bovine somatotropin
  • igf-binding proteins
  • flow-cytometric immunoassay
  • growth-factor-i
  • dexamethasone treatment
  • antibody-formation
  • veal calves
  • insulin
  • serum
  • bone

Cite this

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title = "Potential of Treatment-Specific Protein Biomarker Profiles for Detection of Hormone Abuse in Cattle",
abstract = "Targeted protein biomarker profiling is suggested as a fast screening approach for detection of illegal hormone treatment in meat production. The advantage of using biomarkers is that they mark the biological response and, thus, are responsive to a panel of substances with similar effects. In a preliminary feasibility study, a 4-plex protein biomarker flow cytometric immunoassay (FCIA) previously developed for the detection of recombinant bovine somatotropin (rbST) was applied to cattle treated with steroids, such as estradiol, dexamethasone, and prednisolone. Each treatment resulted in a specific plasma biomarker profile for insulin-like growth factor-1 (IGF-1), IGF binding protein 2, osteocalcin, and anti-rbST antibodies, which could be distinguished from the profile of untreated animals. In summary, the 4-plex biomarker FCIA is, apart from rbST, also capable of detecting treatment with other growth-promoting agents and therefore clearly shows the potential of biomarker profiling as a screening method in veterinary control. It is proposed to perform additional validation studies covering high numbers of treated and untreated animals to support inclusion or adaptation of protein biomarker approaches in future monitoring regulations.",
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Potential of Treatment-Specific Protein Biomarker Profiles for Detection of Hormone Abuse in Cattle. / Ludwig, S.K.J.; Smits, N.G.E.; Cannizzo, F.T.; Nielen, M.W.F.

In: Journal of Agricultural and Food Chemistry, Vol. 61, No. 19, 2013, p. 4514-4519.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Potential of Treatment-Specific Protein Biomarker Profiles for Detection of Hormone Abuse in Cattle

AU - Ludwig, S.K.J.

AU - Smits, N.G.E.

AU - Cannizzo, F.T.

AU - Nielen, M.W.F.

PY - 2013

Y1 - 2013

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AB - Targeted protein biomarker profiling is suggested as a fast screening approach for detection of illegal hormone treatment in meat production. The advantage of using biomarkers is that they mark the biological response and, thus, are responsive to a panel of substances with similar effects. In a preliminary feasibility study, a 4-plex protein biomarker flow cytometric immunoassay (FCIA) previously developed for the detection of recombinant bovine somatotropin (rbST) was applied to cattle treated with steroids, such as estradiol, dexamethasone, and prednisolone. Each treatment resulted in a specific plasma biomarker profile for insulin-like growth factor-1 (IGF-1), IGF binding protein 2, osteocalcin, and anti-rbST antibodies, which could be distinguished from the profile of untreated animals. In summary, the 4-plex biomarker FCIA is, apart from rbST, also capable of detecting treatment with other growth-promoting agents and therefore clearly shows the potential of biomarker profiling as a screening method in veterinary control. It is proposed to perform additional validation studies covering high numbers of treated and untreated animals to support inclusion or adaptation of protein biomarker approaches in future monitoring regulations.

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KW - serum

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