Potato tuber proteomics: Comparison of two complementary extraction methods designed for 2-DE of acidic proteins

P. Delaplace; F. van der Wal; J.F. Dierick; J.H.G. Cordewener; M.L. Fauconnier; P. du Jardin; A.H.P. America

doi:10.1002/pmic.200600493

Potato tuber proteomics: Comparison of two complementary extraction methods designed for 2-DE of acidic proteins

P. Delaplace, F. van der Wal, J.F. Dierick, J.H.G. Cordewener, M.L. Fauconnier, P. du Jardin, A.H.P. America

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Two protein extraction procedures were tested in order to remove interfering compounds prior to 2-DE of potato tubers. These methods using SDS lysis buffer and phenol-phase extraction were compared regarding the quality of the resulting 2-D gel. While the resolution of SDS extracts on semipreparative gels seems better, both methods lead to similar extraction yields and total number of spots. The procedures are complementary regarding the Mr range of preferentially extracted proteins

Original language	English
Pages (from-to)	6494-6497
Journal	Proteomics
Volume	6
Issue number	24
DOIs	https://doi.org/10.1002/pmic.200600493
Publication status	Published - 2006

Keywords

dimensional gel-electrophoresis
recalcitrant plant-tissues
2-dimensional electrophoresis
membrane-proteins
solanum-tuberosum
solubilization
dormancy
storage

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10.1002/pmic.200600493

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title = "Potato tuber proteomics: Comparison of two complementary extraction methods designed for 2-DE of acidic proteins",

abstract = "Two protein extraction procedures were tested in order to remove interfering compounds prior to 2-DE of potato tubers. These methods using SDS lysis buffer and phenol-phase extraction were compared regarding the quality of the resulting 2-D gel. While the resolution of SDS extracts on semipreparative gels seems better, both methods lead to similar extraction yields and total number of spots. The procedures are complementary regarding the Mr range of preferentially extracted proteins",

keywords = "dimensional gel-electrophoresis, recalcitrant plant-tissues, 2-dimensional electrophoresis, membrane-proteins, solanum-tuberosum, solubilization, dormancy, storage",

author = "P. Delaplace and {van der Wal}, F. and J.F. Dierick and J.H.G. Cordewener and M.L. Fauconnier and {du Jardin}, P. and A.H.P. America",

year = "2006",

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T1 - Potato tuber proteomics: Comparison of two complementary extraction methods designed for 2-DE of acidic proteins

AU - Delaplace, P.

AU - van der Wal, F.

AU - Dierick, J.F.

AU - Cordewener, J.H.G.

AU - Fauconnier, M.L.

AU - du Jardin, P.

AU - America, A.H.P.

PY - 2006

Y1 - 2006

N2 - Two protein extraction procedures were tested in order to remove interfering compounds prior to 2-DE of potato tubers. These methods using SDS lysis buffer and phenol-phase extraction were compared regarding the quality of the resulting 2-D gel. While the resolution of SDS extracts on semipreparative gels seems better, both methods lead to similar extraction yields and total number of spots. The procedures are complementary regarding the Mr range of preferentially extracted proteins

AB - Two protein extraction procedures were tested in order to remove interfering compounds prior to 2-DE of potato tubers. These methods using SDS lysis buffer and phenol-phase extraction were compared regarding the quality of the resulting 2-D gel. While the resolution of SDS extracts on semipreparative gels seems better, both methods lead to similar extraction yields and total number of spots. The procedures are complementary regarding the Mr range of preferentially extracted proteins

KW - dimensional gel-electrophoresis

KW - recalcitrant plant-tissues

KW - 2-dimensional electrophoresis

KW - membrane-proteins

KW - solanum-tuberosum

KW - solubilization

KW - dormancy

KW - storage

U2 - 10.1002/pmic.200600493

DO - 10.1002/pmic.200600493

M3 - Article

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VL - 6

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EP - 6497

JO - Proteomics

JF - Proteomics

IS - 24

ER -

Potato tuber proteomics: Comparison of two complementary extraction methods designed for 2-DE of acidic proteins

Abstract

Keywords

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