Potato leafroll virus, its purification from its vector Myzus persicae

D. Peters

Research output: Thesisinternal PhD, WUAcademic

Abstract

Experiments were described on the purification of potato leafroll virus (PLRV) from its vector Myzus persicae. Preliminary experiments demonstrated that much non- viral material had to be removed from aphid macerates to obtain a pure virus preparation. This was achieved by a multistep procedure, in which the aphid macerates were emulsified with chloroform at pH 5.0. The virus present in the interphase between the chloroform and water phases was extracted from the interphase, and concentrated by high-speed centrifuging. The virus suspension obtained was subjected to a phase system of butoxy-ethanol, ethoxy-ethanol and 2.5 M phosphate buffer pH 7.5, and centrifuged in a sucrose density gradient column.

A fairly pure virus preparation was obtained when the material from the infective zone of the gradient was concentrated at 90,000 g for 3 h. Particles with a diameter of 23 mμand with a hexagonal outline were found in each preparation by electron- microscopy. The PLRV isolate purified was free of any contaminating virus when its biological purity was tested. One of the PLRV isolates used in the preliminary experiments, was contaminated with other, presumable persistent, viruses. Their properties and some studies on their host range were described. These viruses were referred to as virus-like particles.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • van der Want, J.P.H., Promotor, External person
  • de Wilde, J., Promotor, External person
Award date6 Oct 1967
Place of PublicationWageningen
Publisher
Publication statusPublished - 1967

Fingerprint

Potato leafroll virus
Myzus persicae
viruses
chloroform
interphase
Aphidoidea
ethanol
virus-like particles
host range
purity
electron microscopy
buffers
sucrose
phosphates

Keywords

  • plant diseases
  • plant viruses
  • solanum tuberosum
  • potatoes
  • plant protection
  • destruction
  • disease vectors
  • insects
  • vector control
  • cum laude

Cite this

Peters, D.. / Potato leafroll virus, its purification from its vector Myzus persicae. Wageningen : Veenman, 1967. 100 p.
@phdthesis{142bd1a4d4a04a70b6d962b1db1145ab,
title = "Potato leafroll virus, its purification from its vector Myzus persicae",
abstract = "Experiments were described on the purification of potato leafroll virus (PLRV) from its vector Myzus persicae. Preliminary experiments demonstrated that much non- viral material had to be removed from aphid macerates to obtain a pure virus preparation. This was achieved by a multistep procedure, in which the aphid macerates were emulsified with chloroform at pH 5.0. The virus present in the interphase between the chloroform and water phases was extracted from the interphase, and concentrated by high-speed centrifuging. The virus suspension obtained was subjected to a phase system of butoxy-ethanol, ethoxy-ethanol and 2.5 M phosphate buffer pH 7.5, and centrifuged in a sucrose density gradient column.A fairly pure virus preparation was obtained when the material from the infective zone of the gradient was concentrated at 90,000 g for 3 h. Particles with a diameter of 23 mμand with a hexagonal outline were found in each preparation by electron- microscopy. The PLRV isolate purified was free of any contaminating virus when its biological purity was tested. One of the PLRV isolates used in the preliminary experiments, was contaminated with other, presumable persistent, viruses. Their properties and some studies on their host range were described. These viruses were referred to as virus-like particles.",
keywords = "plantenziekten, plantenvirussen, solanum tuberosum, aardappelen, gewasbescherming, destructie, vectoren, ziekten, insecten, vectorbestrijding, plant diseases, plant viruses, solanum tuberosum, potatoes, plant protection, destruction, disease vectors, insects, vector control, cum laude",
author = "D. Peters",
note = "WU thesis 414 Proefschrift Wageningen",
year = "1967",
language = "English",
publisher = "Veenman",
school = "Wageningen University",

}

Peters, D 1967, 'Potato leafroll virus, its purification from its vector Myzus persicae', Doctor of Philosophy, Wageningen University, Wageningen.

Potato leafroll virus, its purification from its vector Myzus persicae. / Peters, D.

Wageningen : Veenman, 1967. 100 p.

Research output: Thesisinternal PhD, WUAcademic

TY - THES

T1 - Potato leafroll virus, its purification from its vector Myzus persicae

AU - Peters, D.

N1 - WU thesis 414 Proefschrift Wageningen

PY - 1967

Y1 - 1967

N2 - Experiments were described on the purification of potato leafroll virus (PLRV) from its vector Myzus persicae. Preliminary experiments demonstrated that much non- viral material had to be removed from aphid macerates to obtain a pure virus preparation. This was achieved by a multistep procedure, in which the aphid macerates were emulsified with chloroform at pH 5.0. The virus present in the interphase between the chloroform and water phases was extracted from the interphase, and concentrated by high-speed centrifuging. The virus suspension obtained was subjected to a phase system of butoxy-ethanol, ethoxy-ethanol and 2.5 M phosphate buffer pH 7.5, and centrifuged in a sucrose density gradient column.A fairly pure virus preparation was obtained when the material from the infective zone of the gradient was concentrated at 90,000 g for 3 h. Particles with a diameter of 23 mμand with a hexagonal outline were found in each preparation by electron- microscopy. The PLRV isolate purified was free of any contaminating virus when its biological purity was tested. One of the PLRV isolates used in the preliminary experiments, was contaminated with other, presumable persistent, viruses. Their properties and some studies on their host range were described. These viruses were referred to as virus-like particles.

AB - Experiments were described on the purification of potato leafroll virus (PLRV) from its vector Myzus persicae. Preliminary experiments demonstrated that much non- viral material had to be removed from aphid macerates to obtain a pure virus preparation. This was achieved by a multistep procedure, in which the aphid macerates were emulsified with chloroform at pH 5.0. The virus present in the interphase between the chloroform and water phases was extracted from the interphase, and concentrated by high-speed centrifuging. The virus suspension obtained was subjected to a phase system of butoxy-ethanol, ethoxy-ethanol and 2.5 M phosphate buffer pH 7.5, and centrifuged in a sucrose density gradient column.A fairly pure virus preparation was obtained when the material from the infective zone of the gradient was concentrated at 90,000 g for 3 h. Particles with a diameter of 23 mμand with a hexagonal outline were found in each preparation by electron- microscopy. The PLRV isolate purified was free of any contaminating virus when its biological purity was tested. One of the PLRV isolates used in the preliminary experiments, was contaminated with other, presumable persistent, viruses. Their properties and some studies on their host range were described. These viruses were referred to as virus-like particles.

KW - plantenziekten

KW - plantenvirussen

KW - solanum tuberosum

KW - aardappelen

KW - gewasbescherming

KW - destructie

KW - vectoren, ziekten

KW - insecten

KW - vectorbestrijding

KW - plant diseases

KW - plant viruses

KW - solanum tuberosum

KW - potatoes

KW - plant protection

KW - destruction

KW - disease vectors

KW - insects

KW - vector control

KW - cum laude

M3 - internal PhD, WU

PB - Veenman

CY - Wageningen

ER -