Abstract
The economic value of potato crop resides in its tubers, but most research has focused on foliar resistance to late blight (Phytophthora infestans). Recently, R1 and Rpi-phu1 (Park et al. 2005; Sliwka et al. 2006) conferring resistance to
Phytophthora infestans were shown to confer foliar and tuber resistances whereas R3a and Rpi-abpt were foliage specific. Millett and Bradeen (San Diego 2005) showed that RB gene was constitutively expressed in leaf and tuber but conferred only foliar resistance. These results suggested that not all
R-genes can confer both foliar and tuber resistances. In order to trigger a hypersensitive responses (HR), an Agrobacterium tumefaciens Transient Assay (ATTA) in the tubers was developed. Upon infiltration of IPIO1, Avr2, Avr1 and Avr3a HRs were triggered in transgenic tubers expressing Rpi-blb1,
Rpi-blb3, R1 or R3a. This result showed that all R-genes, conferring tuber resistance or not, could trigger a HR upon effector infiltration. Moreover, during P. infestans infection, IPIO1, Avr2, Avr1 and Avr3a were expressed in leaves and
tubers. Brugmans et al. (2008) performed NBS profiling using cDNA from leaf, stem and root showing different R-gene expression patterns between tissues and between individuals within a particular tissue. We studied the expression patterns of Rpi-blb1, Rpi-blb3, R1, R3a and Rpi-vnt1.1 by QRT-PCR in
leaf and tuber tissues. Comparing the R-gene expression with ability to trigger HR using ATTA, as well as the ability to display resistance to a compatible isolate, we hypothesize that the expression level of a given R-gene may be determinant for tuber resistance. The implications of these results for GMO
applications will be discussed. This work was financially supported by the FP6 programme BioExploit Food-CT-2005-513959.
Phytophthora infestans were shown to confer foliar and tuber resistances whereas R3a and Rpi-abpt were foliage specific. Millett and Bradeen (San Diego 2005) showed that RB gene was constitutively expressed in leaf and tuber but conferred only foliar resistance. These results suggested that not all
R-genes can confer both foliar and tuber resistances. In order to trigger a hypersensitive responses (HR), an Agrobacterium tumefaciens Transient Assay (ATTA) in the tubers was developed. Upon infiltration of IPIO1, Avr2, Avr1 and Avr3a HRs were triggered in transgenic tubers expressing Rpi-blb1,
Rpi-blb3, R1 or R3a. This result showed that all R-genes, conferring tuber resistance or not, could trigger a HR upon effector infiltration. Moreover, during P. infestans infection, IPIO1, Avr2, Avr1 and Avr3a were expressed in leaves and
tubers. Brugmans et al. (2008) performed NBS profiling using cDNA from leaf, stem and root showing different R-gene expression patterns between tissues and between individuals within a particular tissue. We studied the expression patterns of Rpi-blb1, Rpi-blb3, R1, R3a and Rpi-vnt1.1 by QRT-PCR in
leaf and tuber tissues. Comparing the R-gene expression with ability to trigger HR using ATTA, as well as the ability to display resistance to a compatible isolate, we hypothesize that the expression level of a given R-gene may be determinant for tuber resistance. The implications of these results for GMO
applications will be discussed. This work was financially supported by the FP6 programme BioExploit Food-CT-2005-513959.
| Original language | English |
|---|---|
| Title of host publication | ISMPMI International Congress abstracts, Quebec City, Canada, 19-23 July 2009 |
| Publisher | International Society for Molecular Plant-Microbe Interactions |
| Pages | 103 |
| Publication status | Published - 2009 |
| Event | XIV International Congress on Molecular Plant-Microbe Interactions, Quebec City, Canada - Duration: 19 Jul 2009 → 23 Jul 2009 |
Conference
| Conference | XIV International Congress on Molecular Plant-Microbe Interactions, Quebec City, Canada |
|---|---|
| Period | 19/07/09 → 23/07/09 |
