Post alignment clustering procedure for comparative quantitative proteomics LC-MS Data

J.C.W. de Groot, M.W.E.J. Fiers, R.C.H.J. van Ham, A.H.P. America

    Research output: Contribution to journalArticleAcademicpeer-review

    16 Citations (Scopus)

    Abstract

    Comparative LC-MS is a powerful method for detailed quantitative comparison of complex protein mixtures. Dedicated software is required for detection, matching, and alignment of peaks in multiple LC-MS datasets. However, retention time shifts, saturation effects, limitations of experimental accuracy, and possible occurrence of split peaks make it difficult for software to perfectly match all chromatograms. We describe a procedure to assess the above problems and show that dataset quality can be enhanced with the aid of cluster analysis.
    Original languageEnglish
    Pages (from-to)32-36
    JournalProteomics
    Volume8
    Issue number1
    DOIs
    Publication statusPublished - 2008

    Keywords

    • identification
    • proteins

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