Population and molecular genetics of root-knot nematodes

M. Dautova

Research output: Thesisinternal PhD, WU

Abstract

This thesis describes studies of root-knot nematodes Meloidogyne spp. - an economically important pest in agriculture - using population and molecular genetics. Variability in virulence to Mi bearing tomato genotypes is shown for Meloidogyne spp . isolates and their impact of implementation of proper management systems is discussed. Genetic polymorphisms in Meloidogyne spp. were assessed using molecular techniques that reveal nuclear and mitochondrial DNA divergence on different hierarchical levels. cDNA library constructed from the pre-parasitic second stage juveniles of Meloidogyne incognita was screened by single pass 5'-end sequencing to investigate genes expressed at the onset of parasitism. One expressed sequence tag homologous to an endoxylanase was used to identify a novel cell wall degrading enzyme - xylanase. Several lines of evidence support the endogenous origin of the nematode xylanase. The amino acid sequence revealed a high similarity with bacterial xylanases indicating that nematode xylanase may have been acquired from bacteria by horizontal gene transfer.

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Bakker, Jaap, Promotor
  • Gommers, F.J., Promotor, External person
  • Smant, Geert, Promotor
Award date22 Jun 2001
Place of PublicationS.l.
Print ISBNs9789058084286
Publication statusPublished - 2001

Keywords

  • meloidogyne
  • plant parasitic nematodes
  • population genetics
  • molecular genetics

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