Polymorphic microsatellites developed by cross-species amplifications in common pheasant breeds

M. Baratti, A. Alberti, M.A.M. Groenen, T. Veenendaal, F.D. Fulgheri

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)

Abstract

Genetic variability was analysed in two common breeds of pheasant (Phasianus colchicus L. 1758) by means of cross-species amplifications of microsatellite loci: 154 chicken, Gallus gallus and 32 turkey, Meleagris gallopavo, primers were tested for amplification of pheasant DNA. Thirty-six primers (25 specific for chicken and 11 for turkey) amplified pheasant DNA. Fifteen markers yielded specific products and were tested for polymorphism. Eight of them (55€were polymorphic, with an average polymorphism of two alleles per locus. Specific polymerase chain reaction (PCR) products were sequenced; repeats were found in 11 of the 15 markers, although only two loci showed the same repeat and could be homologous to chicken ones.
Original languageEnglish
Pages (from-to)222-225
JournalAnimal Genetics
Volume32
DOIs
Publication statusPublished - 2001

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