Polymerase chain reaction and denaturing gradient gel electrophoresis monitoring of fecal Bifidobacterium populations in a prebiotic and probiotic feeding trial

R.M. Satokari, E.E. Vaughan, A.D.L. Akkermans, W.M. de Vos

Research output: Contribution to journalArticleAcademicpeer-review

78 Citations (Scopus)

Abstract

A culture-independent approach based on genus-specific PCR and denaturing gradient gel electrophoresis (DGGE) was used to monitor qualitative changes in fecal bifidobacterial communities in a human feeding trial. DNA was extracted directly from feces and bifidobacterial 16S rDNA sequences were amplified using genus-specific PCR. The PCR fragments were subsequently separated in a sequence-specific manner by DGGE in order to obtain a profile of bifidobacterial fragments. The DGGE profiles revealed that in general, administration for two weeks of galactooligosaccharide and/or Bifidobacterium lactis Bb-12 (8 g and 3 ́ 1010 cfu per day, respectively) did not affect the qualitative composition of the indigenous Bifidobacterium population, while B. lactis Bb-12 transiently colonised the gut.
Original languageEnglish
Pages (from-to)227-231
JournalSystematic and Applied Microbiology
Volume24
DOIs
Publication statusPublished - 2001

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