Pneumococcal colonization and invasive disease studied in a porcine model

Astrid de Greeff*, Saskia van Selm, Herma Buys, José F. Harders-Westerveen, Rahajeng N. Tunjungputri, Quirijn de Mast, Andre J. van der Ven, Norbert Stockhofe-Zurwieden, Marien I. de Jonge, Hilde E. Smith

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

Background: Streptococcus pneumoniae, a Gram-positive bacterium carried in the human nasopharynx, is an important human pathogen causing mild diseases such as otitis media and sinusitis as well as severe diseases including pneumonia, meningitis and sepsis. There is a strong resemblance between the anatomy, immunology and physiology of the pig and human species. Furthermore, there are striking similarities between S. suis pathogenesis in piglets and S. pneumoniae pathogenesis in humans. Therefore, we investigated the use of piglets as a model for pneumococcal colonization and invasive disease. Results: Intravenous inoculation of piglets with an invasive pneumococcal isolate led to bacteraemia during 5 days, showing clear bacterial replication in the first two days. Bacteraemia was frequently associated with fever and septic arthritis. Moreover, intranasal inoculation of piglets with a nasopharyngeal isolate led to colonization for at least six consecutive days. Conclusions: This demonstrates that central aspects of human pneumococcal infections can be modelled in piglets enabling the use of this model for studies on colonization and transmission but also on development of vaccines and host-directed therapies. Moreover this is the first example of an animal model inducing high levels of pneumococcal septic arthritis.

Original languageEnglish
Article number102
JournalBMC Microbiology
Volume16
Issue number1
DOIs
Publication statusPublished - 2016

Fingerprint

Swine
Infectious Arthritis
Bacteremia
Streptococcus pneumoniae
Pneumococcal Infections
Nasopharynx
Sinusitis
Otitis Media
Gram-Positive Bacteria
Allergy and Immunology
Meningitis
Anatomy
Sepsis
Pneumonia
Fever
Vaccines
Animal Models
Therapeutics

Keywords

  • Animal model
  • Colonization
  • Pigs
  • Streptococcus pneumoniae

Cite this

de Greeff, Astrid ; van Selm, Saskia ; Buys, Herma ; Harders-Westerveen, José F. ; Tunjungputri, Rahajeng N. ; de Mast, Quirijn ; van der Ven, Andre J. ; Stockhofe-Zurwieden, Norbert ; de Jonge, Marien I. ; Smith, Hilde E. / Pneumococcal colonization and invasive disease studied in a porcine model. In: BMC Microbiology. 2016 ; Vol. 16, No. 1.
@article{8cbb41dd907e4742841323d0d41d075d,
title = "Pneumococcal colonization and invasive disease studied in a porcine model",
abstract = "Background: Streptococcus pneumoniae, a Gram-positive bacterium carried in the human nasopharynx, is an important human pathogen causing mild diseases such as otitis media and sinusitis as well as severe diseases including pneumonia, meningitis and sepsis. There is a strong resemblance between the anatomy, immunology and physiology of the pig and human species. Furthermore, there are striking similarities between S. suis pathogenesis in piglets and S. pneumoniae pathogenesis in humans. Therefore, we investigated the use of piglets as a model for pneumococcal colonization and invasive disease. Results: Intravenous inoculation of piglets with an invasive pneumococcal isolate led to bacteraemia during 5 days, showing clear bacterial replication in the first two days. Bacteraemia was frequently associated with fever and septic arthritis. Moreover, intranasal inoculation of piglets with a nasopharyngeal isolate led to colonization for at least six consecutive days. Conclusions: This demonstrates that central aspects of human pneumococcal infections can be modelled in piglets enabling the use of this model for studies on colonization and transmission but also on development of vaccines and host-directed therapies. Moreover this is the first example of an animal model inducing high levels of pneumococcal septic arthritis.",
keywords = "Animal model, Colonization, Pigs, Streptococcus pneumoniae",
author = "{de Greeff}, Astrid and {van Selm}, Saskia and Herma Buys and Harders-Westerveen, {Jos{\'e} F.} and Tunjungputri, {Rahajeng N.} and {de Mast}, Quirijn and {van der Ven}, {Andre J.} and Norbert Stockhofe-Zurwieden and {de Jonge}, {Marien I.} and Smith, {Hilde E.}",
year = "2016",
doi = "10.1186/s12866-016-0718-3",
language = "English",
volume = "16",
journal = "BMC Microbiology",
issn = "1471-2180",
publisher = "Springer Verlag",
number = "1",

}

de Greeff, A, van Selm, S, Buys, H, Harders-Westerveen, JF, Tunjungputri, RN, de Mast, Q, van der Ven, AJ, Stockhofe-Zurwieden, N, de Jonge, MI & Smith, HE 2016, 'Pneumococcal colonization and invasive disease studied in a porcine model', BMC Microbiology, vol. 16, no. 1, 102. https://doi.org/10.1186/s12866-016-0718-3

Pneumococcal colonization and invasive disease studied in a porcine model. / de Greeff, Astrid; van Selm, Saskia; Buys, Herma; Harders-Westerveen, José F.; Tunjungputri, Rahajeng N.; de Mast, Quirijn; van der Ven, Andre J.; Stockhofe-Zurwieden, Norbert; de Jonge, Marien I.; Smith, Hilde E.

In: BMC Microbiology, Vol. 16, No. 1, 102, 2016.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Pneumococcal colonization and invasive disease studied in a porcine model

AU - de Greeff, Astrid

AU - van Selm, Saskia

AU - Buys, Herma

AU - Harders-Westerveen, José F.

AU - Tunjungputri, Rahajeng N.

AU - de Mast, Quirijn

AU - van der Ven, Andre J.

AU - Stockhofe-Zurwieden, Norbert

AU - de Jonge, Marien I.

AU - Smith, Hilde E.

PY - 2016

Y1 - 2016

N2 - Background: Streptococcus pneumoniae, a Gram-positive bacterium carried in the human nasopharynx, is an important human pathogen causing mild diseases such as otitis media and sinusitis as well as severe diseases including pneumonia, meningitis and sepsis. There is a strong resemblance between the anatomy, immunology and physiology of the pig and human species. Furthermore, there are striking similarities between S. suis pathogenesis in piglets and S. pneumoniae pathogenesis in humans. Therefore, we investigated the use of piglets as a model for pneumococcal colonization and invasive disease. Results: Intravenous inoculation of piglets with an invasive pneumococcal isolate led to bacteraemia during 5 days, showing clear bacterial replication in the first two days. Bacteraemia was frequently associated with fever and septic arthritis. Moreover, intranasal inoculation of piglets with a nasopharyngeal isolate led to colonization for at least six consecutive days. Conclusions: This demonstrates that central aspects of human pneumococcal infections can be modelled in piglets enabling the use of this model for studies on colonization and transmission but also on development of vaccines and host-directed therapies. Moreover this is the first example of an animal model inducing high levels of pneumococcal septic arthritis.

AB - Background: Streptococcus pneumoniae, a Gram-positive bacterium carried in the human nasopharynx, is an important human pathogen causing mild diseases such as otitis media and sinusitis as well as severe diseases including pneumonia, meningitis and sepsis. There is a strong resemblance between the anatomy, immunology and physiology of the pig and human species. Furthermore, there are striking similarities between S. suis pathogenesis in piglets and S. pneumoniae pathogenesis in humans. Therefore, we investigated the use of piglets as a model for pneumococcal colonization and invasive disease. Results: Intravenous inoculation of piglets with an invasive pneumococcal isolate led to bacteraemia during 5 days, showing clear bacterial replication in the first two days. Bacteraemia was frequently associated with fever and septic arthritis. Moreover, intranasal inoculation of piglets with a nasopharyngeal isolate led to colonization for at least six consecutive days. Conclusions: This demonstrates that central aspects of human pneumococcal infections can be modelled in piglets enabling the use of this model for studies on colonization and transmission but also on development of vaccines and host-directed therapies. Moreover this is the first example of an animal model inducing high levels of pneumococcal septic arthritis.

KW - Animal model

KW - Colonization

KW - Pigs

KW - Streptococcus pneumoniae

U2 - 10.1186/s12866-016-0718-3

DO - 10.1186/s12866-016-0718-3

M3 - Article

VL - 16

JO - BMC Microbiology

JF - BMC Microbiology

SN - 1471-2180

IS - 1

M1 - 102

ER -