Pluronic-lysozyme conjugates as anti-adhesive and antibacterial bifunctional polymers for surface coating

A.K. Muszanska, H.J. Busscher, A. Herrmann, H.C. van der Mei, W. Norde

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Abstract

This paper describes the preparation and characterization of polymer protein conjugates composed of a synthetic triblock copolymer with a central polypropylene oxide (PPO) block and two terminal polyethylene oxide (PEO) segments, Pluronic F-127, and the antibacterial enzyme lysozyme attached to the telechelic groups of the PEO chains. Covalent conjugation of lysozyme proceeded via reductive amination of aldehyde functionalized PEO blocks (CHO-Pluronic) and the amine groups of the lysine residues in the protein. SDS-PAGE gel electrophoresis together with MALDI-TOF mass spectrometry analysis revealed formation of conjugates of one or two lysozyme molecules per Pluronic polymer chain. The conjugated lysozyme showed antibacterial activity towards Bacillus subtilis. Analysis with a quartz crystal microbalance with dissipation revealed that Pluronic lysozyme conjugates adsorb in a brush conformation on a hydrophobic gold-coated quartz surface. X-ray photoelectron spectroscopy indicated surface coverage of 32% by lysozyme when adsorbed from a mixture of unconjugated Pluronic and Pluronic lysozyme conjugate (ratio 99:1) and of 47% after adsorption of 100% Pluronic-lysozyme conjugates. Thus, bifunctional brushes were created, possessing both anti-adhesive activity due to the polymer brush, combined with the antibacterial activity of lysozyme. The coating having a lower degree of lysozyme coverage proved to be more bactericidal.
Original languageEnglish
Pages (from-to)6333-6341
JournalBiomaterials
Volume32
Issue number26
DOIs
Publication statusPublished - 2011

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Keywords

  • egg-white lysozyme
  • protein adsorption
  • bacterial adhesion
  • block-copolymers
  • hydrophobic surfaces
  • grafting density
  • biomaterials
  • brushes
  • temperature
  • interfaces

Cite this

Muszanska, A. K., Busscher, H. J., Herrmann, A., van der Mei, H. C., & Norde, W. (2011). Pluronic-lysozyme conjugates as anti-adhesive and antibacterial bifunctional polymers for surface coating. Biomaterials, 32(26), 6333-6341. https://doi.org/10.1016/j.biomaterials.2011.05.016