Plasticity of the MFS1 promoter leads to multidrug resistance in the wheat pathogen Zymoseptoria tritici

Selim Omrane, Colette Audéon, Amandine Ignace, Clémentine Duplaix, Lamia Aouini, Gert Kema, Anne Sophie Walker, Sabine Fillinger*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

16 Citations (Scopus)

Abstract

The ascomycete Zymoseptoria tritici is the causal agent of Septoria leaf blotch on wheat. Disease control relies mainly on resistant wheat cultivars and on fungicide applications. The fungus displays a high potential to circumvent both methods. Resistance against all unisite fungicides has been observed over decades. A different type of resistance has emerged among wild populations with multidrugresistant (MDR) strains. Active fungicide efflux through overexpression of the major facilitator gene MFS1 explains this emerging resistance mechanism. Applying a bulkprogeny sequencing approach, we identified in this study a 519-bp long terminal repeat (LTR) insert in the MFS1 promoter, a relic of a retrotransposon cosegregating with the MDR phenotype. Through gene replacement, we show the insert as a mutation responsible for MFS1 overexpression and the MDR phenotype. Besides this type I insert, we found two different types of promoter inserts in more recent MDR strains. Type I and type II inserts harbor potential transcription factor binding sites, but not the type III insert. Interestingly, all three inserts correspond to repeated elements present at different genomic locations in either IPO323 or other Z. tritici strains. These results underline the plasticity of repeated elements leading to fungicide resistance in Z. tritici and which contribute to its adaptive potential.
Original languageEnglish
JournalmSphere
Volume2
Issue number5
DOIs
Publication statusPublished - 1 Sep 2017

Keywords

  • Antifungal resistance
  • Bulk progeny analysis
  • Efflux pumps
  • Multidrug resistance
  • Repeated elements
  • Transcriptional regulation

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  • Research Output

    • 16 Citations
    • 1 Comment/Letter to the editor

    Correction for Omrane et al., "Plasticity of the MFS1 Promoter Leads to Multidrug Resistance in the Wheat Pathogen Zymoseptoria tritici"

    Omrane, S., Audéon, C., Ignace, A., Duplaix, C., Aouini, L., Kema, G., Walker, A. S. & Fillinger, S., 27 Jun 2018, In : mSphere. 3, 3, e00322-18.

    Research output: Contribution to journalComment/Letter to the editorAcademic

    Open Access

    Cite this

    Omrane, S., Audéon, C., Ignace, A., Duplaix, C., Aouini, L., Kema, G., Walker, A. S., & Fillinger, S. (2017). Plasticity of the MFS1 promoter leads to multidrug resistance in the wheat pathogen Zymoseptoria tritici. mSphere, 2(5). https://doi.org/10.1128/mSphere.00393-17