Plasma B vitamins and LINE-1 DNA methylation in leukocytes of patients with a history of colorectal adenomas

A.Y. Jung, A. Botma, C. Lute, H.J. Blom, P.M. Ueland, G. Kvalheim, O. Midttun, F. Nagengast, W.T. Steegenga, E. Kampman

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)


Scope Low concentrations of folate, other B vitamins, and methionine are associated with colorectal cancer risk, possibly by changing DNA methylation patterns. Here, we examine whether plasma concentrations of B vitamins and methionine are associated with methylation of long interspersed nuclear element-1 (LINE-1) among those at high risk of colorectal cancer, i.e. patients with at least one histologically confirmed colorectal adenoma (CRA) in their life. Methods and results We used LINE-1 bisulfite pyrosequencing to measure global DNA methylation levels in leukocytes of 281 CRA patients. Multivariable linear regression was used to assess associations between plasma B vitamin concentrations and LINE-1 methylation levels. Plasma folate was inversely associated with LINE-1 methylation in CRA patients, while plasma methionine was positively associated with LINE-1 methylation. Conclusion This study does not provide evidence that in CRA patients, plasma folate concentrations are positively related to LINE-1 methylation in leukocytes but does suggest a direct association between plasma methionine and LINE-1 methylation in leukocytes.
Original languageEnglish
Pages (from-to)698-708
JournalMolecular Nutrition & Food Research
Issue number4
Publication statusPublished - 2013


  • one-carbon metabolism
  • human methylenetetrahydrofolate reductase
  • moderate folate-depletion
  • tandem mass-spectrometry
  • microbiological assay
  • liquid-chromatography
  • common mutation
  • colon-cancer
  • risk-factor
  • folic-acid


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