Plant growth promotion and induced systemic resistance in tobacco by volatiles from Pseudomonas fluorscens SS101

K. Park, S.H. Lee, M.N. An, K. Balaraju, S.W. Lee, J.E. van de Mortel, J.M. Raaijmakers

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

Plant growth-promoting rhizobacterium (PGPR) can induce various metabolic changes in plants that lead to changes in growth, development and resistance to plant pathogens. Here we show that Pseudomonas fluorscens strain SS101 (Pf.SS101) protects tobacco plants (Nictotiana tobacum L.) from infection by soft rot pathogen Pectobacterim carotovorum SCC1 through induction of systemic resistance (ISR). Strain Pf.SS101 also enhanced biomass of tobacco plants when grown under in vitro conditions using I-plate assay. To determine whether the volatiles from Pf.SS101 are involved in ISR and/or plant growth promotion of tobacco, we performed solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) analysis and bioassays. SPME-GC-MS revealed that Pf.SS101 released at least 12 different volatiles with 13-tetradecadien-1-ol being highly represented. When compared to water-treated control, Pf.SS101 grown on different media showed greater appearance of plant growth promotion and ISR against soft rot in tobacco. However, among various media tested, for the growth of Pf.SS101 to release volatiles for growth promotion and ISR activity, potato dextrose agar (PDA) and King‘s B agar (KBA) media were found to have greater effect on plant growth promotion, while nutrient agar (NA) and KBA were found to have greater effect on ISR activity when compared to tryptic soy agar (TSA), PDA, and luria bertani agar (LBA) media.
Original languageEnglish
Title of host publicationBook of Abstracts 10th International Congress of Plant Pathology, Beijing, China, 25-30 August 2013
Pages257
Volume43(suppl.)
Publication statusPublished - 2013
Event10th International Congress of Plant Pathology: Bio-security, food safety and plant pathology, 25-30 August 2013, Beijing, China -
Duration: 25 Aug 201330 Aug 2013

Conference

Conference10th International Congress of Plant Pathology: Bio-security, food safety and plant pathology, 25-30 August 2013, Beijing, China
Period25/08/1330/08/13

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