Plant Fungal Pathogens: Methods and Protocols

M.D. Bolton (Editor), B.P.H.J. Thomma (Editor)

Research output: Book/ReportBook editingAcademicpeer-review

Abstract

Over the course of evolution, fungi have adapted to occupy specific niches, from symbiotically inhabiting the flora of the intestinal tract of mammals to saprophytic growth on leaf litter resting on the forest floor. In Plant Fungal Pathogens: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study fungal plant pathogens. These include methods and techniques for model systems such as Arabidopsis thaliana as well as crop plants, aspects of fungal biology, genome annotation, next-generation sequencing, and fungal transformation and molecular tools for disease and/or pathogen quantification that are critical for revealing the role for a fungal gene of interest in disease development. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Original languageEnglish
Place of PublicationNew York, USA
PublisherHumana Press
Number of pages648
ISBN (Print)9781617795008
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
PublisherHumana Press, c/o Springer Science+Business Media
No.835

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Keywords

  • plant pathogenic fungi
  • plant-microbe interactions
  • genomics
  • molecular plant pathology
  • fungal diseases
  • methodology
  • protocols

Cite this

Bolton, M. D., & Thomma, B. P. H. J. (Eds.) (2012). Plant Fungal Pathogens: Methods and Protocols. (Methods in Molecular Biology; No. 835). New York, USA: Humana Press. https://doi.org/10.1007/978-1-61779-501-5